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The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript bei

1. The rate of heat production of chemically skinned guinea-pig taenia coli smooth muscle at 25 degrees C was measured using microcalorimetric techniques. 2. Muscle strips were mounted isometrically and incubated in solutions containing MgATP (3.2 mM) and phosphocreatine (PCr, 12 mM), pH 6.9. Activation was obtained by the injection of Ca2+ into the sample compartment of the calorimeter. 3. The he

Eleven arthritic knee joints in seven patients with rheumatoid arthritis were studied before and after intraarticular injection of a corticosteroid preparation. Extensor muscle torque and quantitative electromyography increased on days 7 and 14 after treatment, indicating that muscle function had been inhibited by synovitis. Clinical signs of synovitis, such as pain, range of motion and knee circu

A methodology for the synthesis of novel spiro-cyclohexene bicyclo [2.2.2] octane derivatives, including Claisen rearrangement and ring-closing metathesis (RCM) as key synthetic steps, is described. (c) 2006 Elsevier Ltd. All rights reserved.

We find the extreme programming methodology highly suitable for introducing undergraduate students to software engineering. To be able to apply this methodology at a reasonable teaching cost for large student groups, we have developed two courses that work in tandem: a team programming course taken by more than 100 students, and a coaching course taken by around 25 students. In this paper we descr

The weighted oscillator strengths (gf) and the lifetimes presented in this work were carried out in a multiconfiguration Hartree-Fock relativistic approach. In this calculation, the electrostatic parameters were optimized by a least-squares procedure, in order to improve the adjustment to experimental energy levels. This method produces gf-values that are in better agreement with intensity observa

The aim of this study was to identify ectomycorrhizal species with a potential to release elements from apatite and wood ash and accumulate them in the mycelia. Fungal rhizomorphs and mycelia were sampled from sand-filled mesh bags with or without amendment of apatite or wood ash. The mesh bags were buried in forest soil in the field for 13 or 24 months. Elemental composition of the samples was an

Neuroblastoma is a childhood malignancy originating from cells of the sympathetic nervous system, exhibiting a marked diversity in outcome, with spontaneous regression at one end of the spectrum and severe disease and death at the other end. Features associated with frequent recurrence, a poor prognosis, and high tumor stage are loss of heterozygosity in the distal region of chromosome 1p and ampl

Superficial blood perfusion mas monitored using laser-Doppler perfusion imaging in connection with a phase LII clinical trial comparing photodynamic therapy, utilizing topically applied aminoleuvlinic acid, with cryotherapy of basal cell, carcinomas. A total of 526 images mere recorded before and immediately after the treatment and during the follow-up period. Before treatment, the lesions exhibit

The aim of the study was to evaluate an experimental approach based on a fluid percussion rat trauma model in combination with the microdialysis technique for the analysis of cerebral interstitial biochemical alterations following head trauma, and to test the hypothesis that the previously observed acute accumulation of lactate and increase in the lactate pyruvate ratio may persist for several day

A total of 47 firms joined the Swedish EKO-Energi programme between 1994 and 1997. This was a public voluntary programme devoted to promoting the environmental vanguard, while disregarding the rearguard. The programme offered free energy audits and the promotion of a label, in return for implementation of energy management. Audits covered some 5% of industrial energy consumption. The most clearly

Measurements of Z gamma* production are presented using data collected by the DELPHI detector at centre-of-mass energies ranging from 183 to 209 GeV, corresponding to an integrated luminosity of about 667 pb(-1). The measurements cover a wide range of the possible final state four-fermion configurations: hadronic and leptonic (e(+) e(-) q (q) over bar, mu(+) mu(-) q (q) over bar ,q (q) over barv (

Where am I and what am I seeing? This is a classical vision problem and this paper presents a solution based on efficient use of a combination of 2D and 3D features. Given a model of a scene, the objective is to find the relative camera location of a new input image. Unlike traditional hypothesize-and-test methods that try to estimate the unknown camera position based on 3D model features only, or