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We report on an approach for sulfur dioxide monitoring using broadband absorption spectroscopy in the ultraviolet spectral range. The method was applied in real-time measurements and has the advantage of straightforward data evaluation, limited susceptibility for interference from other gases, and low degree of complexity compared with other real-time optical detection techniques having the same p
Apomorphine is a potent, nonselective, direct-acting dopamine-receptor agonist. Given subcutaneously, it has a rapid onset of antiparkinsonian action qualitatively comparable to that of levodopa. Despite its long history, it was not until peripheral dopaminergic side effects could be controlled by oral domperidone that the clinical usefulness of apomorphine in Parkinson's disease began to be inves
Using literature data on the gas phase reaction between free lanthanide atoms and carbon dioxide, we have found an isokinetic relation for seven of the twelve investigated elements with an isokinetic temperature, T-iso of 2500 +/- 330 K. This value is analyzed in the framework of the model of selective energy transfer, SET, indicating that metal-CO2 complexes are formed as pre-reaction states. The
Despite a high prevalence of persistent groin pain after hernia repair, the specific nature of the pain and its clinical manifestation are poorly known. The aim of this study was to determine the type of post-herniorrhaphy pain and its influence on daily life. In order to assess long-term pain qualitatively and to explore how it affects quality of life, 100 individuals with persisting pain, identi
The PHENIX experiment has measured midrapidity transverse momentum spectra (0.4
We have examined the importance of the actin-based molecular motor myosin 5a for insulin granule transport and insulin secretion. Expression of myosin 5a was downregulated in clonal INS-1E cells using RNAinterference. Stimulated hormone secretion was reduced by 46% and single-cell exocytosis, measured by capacitance recordings, was inhibited by 42% after silencing. Silencing of Slac-2c/MYRIP, whic
Background Long-chain polyunsaturated fatty acid (LCPUFA) especially the n-3-FA of skeletal muscle phospholipids may facilitate insulin action, whereas saturated and trans-FA act oppositely. Community studies show that non-diabetic weight matched obese men and women display similar insulin resistance, despite the fact that an android fat distribution is detrimental to insulin action. The increased
We have exploited the spatial and kinetic resolution of ellipsometry to monitor the lateral movement of inhomogeneous patches of material in mixed adsorption layers of poly(ethylene imine) and sodium dodecyl sulfate at the air/liquid interface. We show that the choice of sample preparation methods can have a profound effect on the state of the interface for chemically equivalent samples. The exten
Delayed contrast-enhanced MRI of cartilage (dGEMRIC) is a noninvasive technique to study cartilage glycosaminoglycan (GAG) content in vivo. This study evaluates dGEMRIC in patients with preradiographic degenerative cartilage changes. Seventeen knees in 15 patients (age 35-70) with arthroscopically verified cartilage changes (softening and fibrillations) in the medial or lateral femoral compartment
Process parameters for the extraction of glycolipids by scCO(2) from Lactobacillus plantarum B-01 were optimized by carrying out a two-level fractional design experiment. The highest glycolipids extract yield was obtained at 250 bar, 40 degrees C, CO2 flow rate, 10 g/min and hydrometanolic co-solvent, 9.3% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids pho
BACKGROUND: Inflammatory mediators secreted by leukocytes are implicated in atherogenesis. Chlamydia (C.) pneumoniae infection has been suggested as a trigger of this process. We investigated relationships between C. Pneumoniae serology, inflammatory mediators and symptomatic cardiovascular disease in old age. METHODS: In a cross-sectional study at baseline with a prospective 4 year follow-up, int
The volume of fluid (VOF) and immersed boundary (IB) methods are two popular computational techniques for multi-fluid dynamics. To help shed light on the performance of both techniques, we present accuracy assessment, which includes interfacial geometry, detailed and global fluid flow characteristics, and computational robustness. The investigation includes the simulations of a droplet under stati
Background: There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. Methods: Transferrin was iron-saturated by mixing the serum with ferric nitrilotriacetic acid, and lipoproteins were precipitated wi
The Northern and Western dialects of the Mon-Khmer language Kammu use fundamental frequency to distinguish words, while the Eastern dialect relies on the contrastive voicing of initial consonants to distinguish words which display an F0 contrast in the other dialects. The production and perception of tones in the three dialects is investigated, using recordings and perception tests. As expected, N
A new concept for affinity two-phase partitioning was tested. The partitioning was based on the interaction of target membranes with a primary antibody which, in turn, interacted with a biotinylated secondary antibody and NeutrAvidin-dextran in a poly(ethylene glycol)/dextran two-phase system. Caveolae selectively redistributed from the top phase to the NeutrAvidin-dextran-containing bottom phase
The contents of short-chain fatty acids were investigated in rats fed lactitol, lactulose and four fructo-oligosaccharides of different degree of polymerisation and solubility. Fructo-oligosaccharides with a low degree of polymerisation (2-8) generated the highest levels of butyric acid all along the hindgut, whereas fructo-oligosaccharides with a high degree of polymerisation (10-60) generated th
Apolipoprotein M (apoM) is a novel apolipoprotein with unknown function. In this study, we established a method for isolating apoM-containing lipoproteins and studied their composition and the effect of apoM on HDL function. ApoM-containing lipoproteins were isolated from human plasma with immunoaffinity chromatography and compared with lipoproteins lacking apoM. The apoM-containing lipoproteins w
OBJECTIVE: Mapping coreceptor epitopes used by the prototypic R5 and X4 strains, HIV-1BaL and HIV-1IIIB, in comparison with epitopes involved in the activation and signaling induced by the natural ligands, RANTES and SDF-1beta. DESIGN: Receptor hybrids between CCR5 and CXCR4 were constructed. METHODS: Using single-overlap and extension PCR, increasing portions of CCR5 were replaced with correspond
