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Heart failure is a global health concern whose burden is increasing as our population ages. Early diagnosis and accurate prognosis are critical for improving outcomes, highlighting the need for novel methods that can better identify and elucidate the pathophysiology of heart failure. The cardinal symptom of heart failure is exercise intolerance, yet most cardiac imaging is performed at rest, poten
Multiple studies have demonstrated that cancer cells with microsatellite instability (MSI) are intolerant to loss of the Werner syndrome helicase (WRN), whereas microsatellite-stable (MSS) cancer cells are not. Therefore, WRN represents a promising new synthetic lethal target for developing drugs to treat cancers with MSI. Given the uncertainty of how effective inhibitors of WRN activity will prov
Histone chaperones control nucleosome density and chromatin structure. In yeast, the H3-H4 chaperone Spt2 controls histone deposition at active genes but its roles in metazoan chromatin structure and organismal physiology are not known. Here we identify the Caenorhabditis elegans ortholog of SPT2 (CeSPT-2) and show that its ability to bind histones H3-H4 is important for germline development and t
Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining 1 . HR supresses tumorigenesis 1 , but is restricted to the S and G2 phases of the cell cycle when a sister chromatid
Homologous recombination (HR) is an important route for repairing DNA double-strand breaks (DSBs). The early stages of HR are well understood, but later stages remain mysterious. In this issue of Genes & Development, Hustedt and colleagues (pp. 1397–1415) reveal HROB as a new player in HR required for recruitment of the MCM8–9 complex, which is paralogous to the MCM2–7 replicative helicase. HR
DNA sequence and epigenetic information embedded in chromatin must be faithfully duplicated and transmitted to daughter cells during cell division. However, how chromatin assembly and DNA replication are integrated remains unclear. We examined the contribution of the Tousled-like kinases 1 and 2 (TLK1/TLK2) to chromatin assembly and maintenance of replication fork integrity. We show that TLK activ
After DNA replication, chromosomal processes including DNA repair and transcription take place in the context of sister chromatids. While cell cycle regulation can guide these processes globally, mechanisms to distinguish pre- and post-replicative states locally remain unknown. Here we reveal that new histones incorporated during DNA replication provide a signature of postreplicative chromatin, re
During DNA replication, chromatin is reassembled by recycling of modified old histones and deposition of new ones. How histone dynamics integrates with DNA replication to maintain genome and epigenome information remains unclear. Here, we reveal how human MCM2, part of the replicative helicase, chaperones histones H3-H4. Our first structure shows an H3-H4 tetramer bound by two MCM2 histone-binding
Genomgång av källäget rörande ett förment citat av Sten Broman.
This thesis is focused on the investigation of the ultrafast structural response of the phase change material (PCM) Ge$_2$Sb$_2$Te$_5$ following laser excitation. Phase change materials are a group of materials capable of rapid, non-volatile, switching between an amorphous and a crystalline phase. The two phases exhibit high optical and electrical contrast, meaning the optical reflectivity and the
Kulturarv har blivit en alltmer politiserad fråga och väcker debatt om tillhörighet, inkludering och identitet, inte minst i relation till nationen. Samtidigt betonas kulturarvens betydelse i frågor som rör lärande, digitalisering, tillväxt, innovation och hållbarhet.I denna antologi undersöker författarna samspelet mellan kulturarvspedagogik och politik inom olika delar av kulturarvssektorn – sås
BACKGROUND: Catheterization of the vein is frequently a painful procedure. Assessing venous cannulation induced pain (VCP) before surgery have been used to predict the intensity of postoperative pain and postoperative opioid consumption. The aim of this study was to investigate the intra-individual variation in venous cannulation induced pain at three consecutive time points and factors influencin
Understanding the drivers of diversity and change in regional Neolithization trajectories has emerged as a major theme in World Prehistory with new insights appearing in many parts of the globe. In Northeast Asia, research on Neolithic transition over the past two decades has focused primarily on millet and rice domestication and their role in the rise of farming, social complexity, and early Chin
Avhandlingen undersöker föreningar i Sverige som organiserar öppna verkstäder. Öppna verkstäder, vilka också kallas för makerspaces, är platser som möjliggör skapande. Verktyg, maskiner och kunskap är delade mellan användarna. Syftet med avhandlingen är att undersöka hur föreningar organiserar sina delade verkstäder, vilka svårigheter föreningarna möter samt vilka implikationer detta har för den bAlternative building as an interstitial mode of transformation plays a central role in the discussion of strategies for degrowth. In this debate the questions of how alternative projects can be scaled up and how degrowth politics can gain broad societal support have been identified as crucial. In this context open workshops – grassroots infrastructures for creative activities, such as repair, upcy
Aim: In uncertain clinical situations, such as assessing the risk of exacerbation of apical periodontitis in root- filled teeth, un-dergraduate students can benefit from being trained to reflect. This study aimed to explore how students specifically reflectedon clinical experiences in relation to uncertainty when assessing a case involving a root- filled tooth with apical periodontitis.Methodolo
