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Investigation of the enzyme Bacillus agaradhaerens Cel 5a as an analytical tool in mass spectral characterisation of methylcelluloses

Two methylcelluloses (MC) were investigated by partial hydrolysis followed by direct infusion electrospray ionisation mass spectrometry (ESI-MS). Partial hydrolysis was achieved either enzymatically or by acid hydrolysis. Enzyme hydrolysis was performed using the endoglucanase Bacillus agaradhaerens Cel 5a. Three different hydrolysis buffer solutions were investigated in order to deter-mine which

On the Analysis and Fault-Diagnosis Tools for Small-Scale Heat and Power Plants

Popular Abstract in Swedish Avregleringen av elmarknaden uppmuntrar kraftbolag och oberoende kraftproducenter till att driva sina kraftverksanläggningar på ett marknadsorienterat sätt. För att behålla de ekonomiska marginalerna på plussidan så har de mest populära åtgärderna varit att förbättra elverkningsgraden genom ändringar i hårdvaran samt höja totalverkningsgraden genom till exempel samproduThe deregulation of the electricity market drives utilities and independent power producers to operate heat and power plants as profit centers. In order to keep the economic margins on the credit side, the preferred measures have been to improve the electrical efficiency through changes in the hardware and boost the overall efficiency through e.g. combined heat and power (CHP) generation. The bett

Intramolecular dynamics of low molecular weight protein tyrosine phosphatase in monomer-dimer equilibrium studied by NMR: A model for changes in dynamics upon target binding

Low molecular weight protein tyrosine phosphatase (LMW-PTP) dimerizes in the phosphate-bound state in solution with a dissociation constant of K-d = 1.5(+/-0.1) mM and an off-rate on the order of 10(4) s(-1).H-1 and N-15 NMR chemical shifts identify the dimer interface, which is in excellent agreement with that observed in the crystal structure of the dimeric S19A mutant. Two tyrosine residues of

Nateglinide Improves Early Insulin Secretion and Controls Postprandial Glucose Excursions in a Prediabetic Population.

OBJECTIVE—The purpose of this study was to evaluate the metabolic effectiveness, safety, and tolerability of nateglinide in subjects with impaired glucose tolerance (IGT) and to identify a dose appropriate for use in a diabetes prevention study. RESEARCH DESIGN AND METHODS—This multicenter, double-blind, randomized, parallel-group, fixed-dose study of 8 weeks’ duration was performed in a total of

Buoyancy Effects on Developing Laminar Gas Flow and Heat Transfer in a Rectangular Fuel Cell Duct

Using a general 3-D finite-volume code, the development of buoyancy force driven secondary flow and its effects on developing laminar flow and heat transfer have been numerically simulated for a horizontal fuel cell duct with a rectangular cross section. The constant thermal-properties assumption except for linear density variation with temperature in the body force term is applied, and a constant

Measuring GFR: A Systematic Review.

Background No comprehensive systematic review of the accuracy of glomerular filtration rate (GFR) measurement methods using renal inulin clearance as reference has been published. Study Design Systematic review with meta-analysis of cross-sectional diagnostic studies. Setting & Population Published original studies and systematic reviews in any population. Selection Criteria for Studies Index

Biomarkers of rapid chronic kidney disease progression in type 2 diabetes.

Here we evaluated the performance of a large set of serum biomarkers for the prediction of rapid progression of chronic kidney disease (CKD) in patients with type 2 diabetes. We used a case-control design nested within a prospective cohort of patients with baseline eGFR 30-60 ml/min per 1.73 m(2). Within a 3.5-year period of Go-DARTS study patients, 154 had over a 40% eGFR decline and 153 controls

A novel feeding strategy for industrial fed-batch processes based on frequency content analysis

Overflow metabolism, i.e. the production of metabolic by-products at a high glycolytic flux, is a recurring problem in fed-batch processes with many types of microorganisms. In the current study, a novel feeding strategy aimed at avoiding process failures due to overflow by-product formation was designed and implemented in a pilot-scale reactor (0.5 m3). The basic principle behind the strategy was

Enzyme-aided investigation of the substituent distribution in cationic potato amylopectin starch

The distribution of substituents along the polymer chain in cationic potato amylopectin starch, modified in solution, granular slurry, or dry state, was investigated. The starch derivatives were successively hydrolyzed by different enzymes, followed by characterization of the hydrolysis products obtained by means of electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ioniz

Urban mesh ad hoc networks

Mesh networking is currently gaining much attention, within both academia and industry. Mesh networking allows cheap and fast deployment of wireless services. It is regarded as a very promising solution for urban deployment scenarios as well as for temporary emergency response situations. Another related promising field is that of ad hoc wireless networking, which consists of mobile nodes that dyn

Development and validation of a lifestyle-based model for colorectal cancer risk prediction : the LiFeCRC score

Background: Nutrition and lifestyle have been long established as risk factors for colorectal cancer (CRC). Modifiable lifestyle behaviours bear potential to minimize long-term CRC risk; however, translation of lifestyle information into individualized CRC risk assessment has not been implemented. Lifestyle-based risk models may aid the identification of high-risk individuals, guide referral to sc

Efficient real-time radial distortion correction for UAVs

In this paper we present a novel algorithm for onboard radial distortion correction for unmanned aerial vehicles (UAVs) equipped with an inertial measurement unit (IMU), that runs in real-time. This approach makes calibration procedures redundant, thus allowing for exchange of optics extemporaneously. By utilizing the IMU data, the cameras can be aligned with the gravity direction. This allows us

Communicating Alcohol Narratives: Creating a Healthier Relationship with Alcohol

Alcohol, like mental health, is a neglected topic in public health discussions. However, it should be defined as a priority public health area because the evidence available to support this is very persuasive. Although only half the world's population drinks alcohol, it is the world's third leading cause of ill health and premature death, after low birth weight and unsafe sex, and the world's grea

Chronic kidney disease after heart transplantation: a single-centre retrospective study at skåne university hospital in lund 1988-2010.

We aimed to study the incidence, predictors, and outcome of chronic kidney disease (CKD) after heart transplantation (HT). All our HT patients 1988-2010 were considered for inclusion. Of these, 134 came for annual follow-ups including evaluation of glomerular filtration rate (GFR) using iohexol clearance measurements, and the CKD-EPI (adults) or Schwartz (children) formulae. Median GFR (Q1-Q3)(mL/

Radiocesium in the forest and forest industry, Studies on the flow, occurrence and technological enhancement of radionuclides in the forest industry with emphasis on radiocesium.

In the aftermath of the nuclear weapons testing and as compared with agriculture, the forest industry has received relatively little attention in spite of its large-scale use of radiocesium- contaminated wood and water. After the Chernobyl nuclear power plant accident in 1986, numerous studies of the forest environment have been conducted with respect to radionuclide transfer through the forest ec

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Extrusion is used to produce crunchy expanded foods, such as snacks. The nutritional impact of this process has not been studied sufficiently. In this study, in vitro and in vivo protein and starch bioavailability was evaluated in both raw and extruded corn (Zea mays)(C) and lima bean (Phaseolus lunatus)(B) flour blends, prepared in 75C/25B and 50C/50B (p/p) proportions. These were processed with

The dry season intensity as a key driver of NPP trends

We analyze the impacts of changing dry season length and intensity on vegetation productivity and biomass. Our results show a wetness asymmetry in dry ecosystems, with dry seasons becoming drier and wet seasons becoming wetter, likely caused by climate change. The increasingly intense dry seasons were consistently correlated with a decreasing trend in net primary productivity (NPP) and biomass fro