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Mitochondrial genome from the facultative anaerobe and petite-positive yeast Dekkera bruxellensis contains the NADH dehydrogenase subunit genes

The progenitor of the Dekkera/Brettanomyces clade separated from the Saccharomyces/Kluyveromyces clade over 200 million years ago. However, within both clades, several lineages developed similar physiological traits. Both Saccharomyces cerevisiae and Dekkera bruxellensis are facultative anaerobes; in the presence of excess oxygen and sugars, they accumulate ethanol (Crabtree effect) and they both

Uncalibrated Structure from Motion on a Sphere

Spherical motion is a special case of camera motion where the camera moves on the imaginary surface of a sphere with the optical axis normal to the surface. Common sources of spherical motion are a person capturing a stereo panorama with a phone held in an outstretched hand, or a hemi-spherical camera rig used for multi-view scene capture. However, traditional structure-from-motion pipelines tend

Exploring the Potential to Improve Photodynamic Therapy of Skin Cancer by Exogenous Catalase and m-ALA Delivery Using a Crystalip® Topical Cream Formulation

Förbättring av Krämer för Fotodynamisk Behandling av Hudcancer Fotodynamisk terapi (PDT) är en modern och relativt skonsam behandlingsmetod som används vid vissa typer av hudcancer och förstadier till hudcancer. I stället för att operera bort vävnad används ett ljuskänsligt läkemedel som appliceras på huden. När det behandlade området belyses med ljus av en specifik våglängd reagerar läkemedlet mePhotodynamic therapy (PDT) is an established treatment for actinic keratosis and selected non-melanoma skin cancers. Still, its broader use is limited by pain, skin irritation, and formulation constraints associated with high photosensitiser concentrations. This study aimed to develop and evaluate a Crystalip®-based topical PDT formulation that enables two-step manufacturing, delivers methyl-amino

Bjurkärr – en småländsk bokskog med unika lavar och svampar.

The old beech forest (250–300 yr) at Bjurkärr (core area 30 ha) in the province of Småland, S Sweden, was surveyed for epiphytic lichens of conservation concern in 1993–94. Several rare red-listed lichens were recorded, many with large populations. In 2011 the most endangered lichens were resurveyed due to the incorporation of Bjurkärr in the forthcoming national park of Lake Åsnen. Most species o

Mothers, Play and Everyday Life: Ethnology Meets Game Studies

This article should serve as an introduction to a relatively new topic in ethnological studies requiring very specific methods as it involves both offline and online research as well as material objects and immaterial practices. How do we embark on an interdisciplinary venture such as this, and be sure to produce qualitative research of high standard? How should gaming mothers best be studied? In

Targeting T cell migration in inflammatory bowel disease.

Crohn's disease and ulcerative colitis are chronic inflammatory disorders of the gastrointestinal tract, and are collectively referred to as inflammatory bowel disease (IBD). IBD is a major cause of lifetime morbidity, has a severe impact on quality of life of patients (equivalent to that of rheumatoid arthritis, asthma, migraine, or diabetes) and constitutes a substantial economic burden to the h

Nature and significance of endoleaks and endotension: summary of opinions expressed at an international conference

OBJECTIVE: Endoleaks and endotension are critically important complications of some endovascular aortic aneurysm repairs (EVARs). For the resolution of controversial issues and the determination of areas of uncertainty relating to these complications, a conference of 27 interested leaders was held on November 20, 2000. METHODS: These 27 participants (21 vascular surgeons, five interventional radio

Benchmark simulation model no 2: general protocol and exploratory case studies

Over a decade ago, the concept of objectively evaluating the performance of control strategies by simulating them using a standard model implementation was introduced for activated sludge wastewater treatment plants. The resulting Benchmark Simulation Model No 1 (BSM1) has been the basis for a significant new development that is reported on here: Rather than only evaluating control strategies at t

Thermal models of buildings : determination of temperatures, heating and cooling loads : theories, models and computer programs

The need to estimate indoor temperatures, heating or cooling load and energy requirements for buildings arises in many stages of a buildings life cycle, e.g. at the early layout stage, during the design of a building and for energy retrofitting planning. Other purposes are to meat the authorities requirements given in building codes. All these situations require good calculation methods. The main

Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11

Background: Loss of chromosome 11q defines a subset of high-stage aggressive neuroblastomas. Deletions are typically large and mapping efforts have thus far not lead to a well defined consensus region, which hampers the identification of positional candidate tumour suppressor genes. In a previous study, functional evidence for a neuroblastoma suppressor gene on chromosome 11 was obtained through m

A platform for Packaging Logistics Development: a systems approach

Packaging Logistics is a concept that recognises the need for coordination of the packaging system and its manifold functions throughout a package’s life cycle. Packaging Logistics is an area in need for substantial theoretical building. This dissertation elaborates on its underpinnings and suggests conceptual models that should be useful in better understanding the core of the concept, its undere

ApoA-I Milano stimulates lipolysis in adipose cells independently of cAMP/PKA activation.

ApoA-I, the main protein component of high-density lipoprotein (HDL), is suggested to be involved in metabolic homeostasis. We examined the effects of Milano, a naturally occurring ApoA-I variant, about which little mechanistic information is available. Remarkably, high fat-fed mice treated with Milano displayed a rapid weight loss greater than ApoA-I WT treated mice, and a significantly reduced a

Human3.6M: Large Scale Datasets and Predictive Methods for 3D Human Sensing in Natural Environments.

We introduce a new dataset, Human3.6M, of 3.6 Million 3D Human poses, acquired by recording the performance of 11 subjects, under 4 different viewpoints, for training realistic human sensing systems and for evaluating the next generation of human pose estimation models. Besides increasing the size the current state of the art datasets by several orders of magnitude, we aim to complement such datas

High resolution scanning gate microscopy measurements on InAs/GaSb nanowire Esaki diode devices

Gated transport measurements are the backbone of electrical characterization of nanoscale electronic devices. Scanning gate microscopy (SGM) is one such gating technique that adds crucial spatial information, accessing the localized properties of semiconductor devices. Nanowires represent a central device concept due to the potential to combine very different materials. However, SGM on semiconduct

A generalized birthday approach for efficiently finding linear relations in l-sequences

Feedback with carry shift registers (FCSRs) have previously been available in two configurations, the Fibonacci and Galois architectures. Recently, a generalized and unifying FCSR structure and theory was presented. The new ring FCSR model repairs some weaknesses of the older architectures. Most notably, the carry cell bias property that was exploited for an attack on the eSTREAM final portfolio c

Numerical analysis of ballistic imaging for revealing liquid breakup in dense sprays

This work demonstrates the capacity of a ballistic imaging instrument to suppress diffuse photons and improve image contrast, making it possible to view fluid structure in a spray where a fog of droplets occludes the near field. Analysis of the system is performed by means of a numerical system model. The model simulates light propagation and scattering in the measurement volume using a Monte Carl

Identification of a trypsin-like serine protease from Trichoderma reesei QM9414

In the present work the genetically modified Trichoderma reesei strain QM9414 was used to produce full-length Ce17B (endoglucanase I, EGI) under the control of the constitutive Aspergillus nidulans gpdA promoter in the presence of glucose. However, the full-length Ce17B enzyme was found to be truncated to lower molecular weight components in the culture broth. Truncation of recombinant proteins pr

Captured metagenomics: large-scale targeting of genes based on 'sequence capture' reveals functional diversity in soils.

Microbial enzyme diversity is a key to understand many ecosystem processes. Whole metagenome sequencing (WMG) obtains information on functional genes, but it is costly and inefficient due to large amount of sequencing that is required. In this study, we have applied a captured metagenomics technique for functional genes in soil microorganisms, as an alternative to WMG. Large-scale targeting of fun

Pollinator attraction to volatiles from virgin and pollinated host flowers in a yucca/moth obligate mutualism

The classic obligate pollination–seed consumption mutualism between yuccas and yucca moths has been thought to be mediated by chemical cues, but empirical data on pollinator attraction to host floral volatiles in this association have been lacking. Here we show that the scent from virgin flowers of the host Yucca glauca is sufficient to attract its obligate pollinator Tegeticula yuccasella in Y-tu