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The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was ab
Several interactions of cytokines with extracellular matrix molecules are mediated by proteoglycans, such as biglycan and decorin. Using surface plasmon resonance, we show for the first time that tumour necrosis factor-alpha (TNF-alpha) binds to both biglycan and decorin with K(d)s of 0.81 microM and 1.23 microM respectively, a binding that was confirmed by Scatchard plots using a solid phase assa
The feasibility of 17 beta-estradiol (E2) and 17 alpha-ethynylestradiol (EE2) removal by Trametes versicolor was demonstrated in batch and continuous cultures. In batch, E2 and EE2 initially supplied at 10 mg l(-1) were removed by more than 97% in 24 h, which corresponded to volumetric removal rates of 0.43 and 0.44 mg l(-1) h(-1), respectively. A bioreactor inoculated with T versicolor pellets wa
This article analyses some young men’s specific ways of retelling victims’ experience when the perpetrators have an immigrant background. In qualitative interviews with twenty young men, fear of being seen as intolerant was distinguished by their expressive caution. This involved using various aligning actions: presenting disclaimers and accounts, but also in terms of hesitant speak, re-takes and This article analyses some young men’s specific ways of retelling victims’ experience when the perpetrators have an immigrant background. In qualitative interviews with twenty young men, fear of being seen as intolerant was distinguished by their expressive caution. This involved using various aligning actions: presenting disclaimers and accounts, but also in terms of hesitant speak, re-takes and
This study describes a method to formulate struvite fine powder into pellets that are easy to spread on agricultural land. To evaluate the quality of produced pellets, some chemical and physical properties commonly measured for fertilizers were tested. The findings indicated that the salt index and heavy metal content ofstruvite pellets were significantly lower than those of commercial NPK fertili
We present an experimental and theoretical study of the F-2(5/2) -> F-2(7/2) M1 transition in Ag-like W (W27+). The experiments employed the Shanghai permanent magnet electron beam ion trap, which has been developed especially for assisting spectroscopic diagnostics of edge plasmas for magnetic fusion devices. The theoretical value was obtained using the GRASP2K set of computer codes and included
We studied the solubilization of oil in the C16E8/hexadecane/H2O system. Close to the phase inversion temperature (PIT), the system, at equilibrium, can form either homogeneous states (i.e., microemulsions) at high surfactant concentrations or three-phase states at lower concentrations. We show that, under gentle shear, at a line we named the clearing boundary (CB), located a few degrees below the
The transition quadrupole moments Q(t) for the superdeformed band in Ca-40 have been determined through thin-target Doppler-shift attenuation analyses. A best-fit value of Q(t)=1.30+/-0.05 e b is obtained when a single value is assumed for the entire band. Fitting separate quadrupole moments for in-band transitions decaying from the high-spin states and the presumably admixed low-spin states resul
Twenty-six plant species from the native flora used in Bolivian folk medicine were selected for studies of bioactivity. They belonged to the following families namely: Asteraceae (11), Brassicaceae (1), Cactaceae (1), Caesalpinaceae (2), Chenopodiaceae (1), Frankeniaceae (1), Geraniaceae (1), Laureaceae (1), Oxalidaceae (1), Piperaceae (1), Plantaginaceae (1), Rosaceae (1), Solanaceae (1) and Verb
We investigated intracapsular pressure and elasticity of the hip joint capsule in osteoarthritis. HOOS hip score, sonography of the hip joint, and radiographic assessment of osteoarthritis were performed in 31 patients before total hip arthroplasty. The intracapsular hydrostatic pressure was measured perioperatively, and 0.9% saline solution was then injected in the hip joint, 1 mL at a time with
Disperse dyes are the most common contact sensitizers among textile dyes. The main aim of this study was to investigate the outcome of patch testing with a textile dye mix 6.6%. A total of 2,049 patients from Sweden and 497 from Belgium were tested with the mix, consisting of Disperse (D) Blue 35, D Yellow 3, D Orange 1 and 3, D Red 1 and 17, 1.0% each, and D Blue 106 and D Blue 124, 0.3% each. Of
BACKGROUND: Certain immune-mediated diseases (IMDs), such as rheumatoid arthritis and systemic lupus erythematosus, have been linked to cardiovascular disorders. We examined whether there is an association between 32 different IMDs and risk of subsequent hospitalization for coronary heart disease (CHD) related to coronary atherosclerosis in a nationwide follow up study in Sweden. METHODS AND FIND
This thesis aimed to examine the relationship between climate and atmospheric CO2 concentrations through rapid climate changes during the Holocene. Climate was reconstructed using high-resolution, radiocarbon dated, lake sediment geochemical proxies (magnetic susceptibility, loss-on-ignition, total carbon/nitrogen/sulphur determinations and biogenic silica), vegetational reconstruction (pollen ana
A novel calcium-independent serine protease from an alkaliphilic bacterium, Nesterenkonia sp. AL20, has been purified and crystallized at 296 K using sodium formate as the main precipitant. This enzyme is optimally active at pH 10, exhibits high stability towards autolytic digestion and its stability is not affected by the presence of EDTA or detergents. The triangular prism-shaped crystals diffra
