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The 65 kDa isoform of human glutamate decarboxylase (GAD65) is a major autoantigen in type 1 diabetes (T1D). In the present study, we have developed a sensitive sandwich time-resolved fluorescence immunoassay (TRFIA) for the quantification of GAD65 in cell extracts, cell media and serum. The monoclonal antibody GAD-6 is used to selectively capture GAD65 but not the slightly larger isoform GAD67, a

This paper presents a fast, interactive and easily modifiable software tool for robust PID design. The Matlab based program is supposed to give people with moderate knowledge on PID control a possibility to learn more and also be a future part of an autotuner. The PID design is made by minimizing the integrated absolute error value during a load disturbance on the process input. The optimization i

Innovations should create value for increasingly individualistic consumers with varying demands and for other stakeholders. Today, retailers have the power in the supply/value chain. This research investigates how Swedish food retailers view innovations, their role and that of customers and suppliers in the development process, and how they see future development. The study is based on open-ended

Problems related to content of fat, oil and grease (FOG) in wastewater is an increasing problem in Sweden. FOG can cause clogging in the sewage system and subsequent flooding and disturbance of processes in waste water treatment plants. The problem has previously been mostly been related to release of large quantities of FOG from restaurants and large scale industries which has caused problems in

This glossary was prepared under the guidance of Hans-Erik Nissen in collaboration with the authors of the papers in the monograph. It is a glossary of terms used in monograph Double Helix relationships in use and design of Informing Systems: Lessons to learn from phenomenology and hermeneutics

Döden är ett evigt tema. Men diskursen runt döden i dess mångskiftande former är underkastad ständig förändring. Döden kan ses som ett hot och ett gissel men också som en befrielse, ett straff eller som ett redskap för att befria samhället från oönskade individer. I samtliga fall kan man tala om döden som en reningsprocess – som katharsis. Det är denna föränderliga diskurs som tas upp i denna bok.

This article proposes to focus on the importance of epistolary mediation as driving force in the construction of scientific knowledge. It is by analysing the correspondence between phoneticians approached as a textual interaction, considered in its mediatory function and captured through the scientific challenges which permeate it, that we shall illustrate here the contribution of the exchange of

In today’s highly competitive market place it is of great importance for companies to deliver reliable products while decreasing the development time and costs. The time to market is a driving force for many companies, and throughout the engineering design process as well as the manufacturing process, the focus is on finding timesaving actions. However, the search for timesaving actions will most

We report detections of weak emission lines in main sequence B starspectra observed at high spectral resolution and high signal-to-noiseratio. The emission lines originate from highly excited states ofsingle-ionized species. The mechanism for populating these excitedstates is currently under investigation and the results may have asignificant bearing upon our understanding of the outermost atmosph

A frequency synthesizer applied to 1000Base-T Ethernet transceiver as well as 10/100Base-T mode is described. A dynamic voltage-mode phase interpolator is used and a more precise analysis and calculation on degressive interpolating resistors are given. The design not only meets the transmitter's requirement of very accurate rising (falling) edge control but also offers much finer time-interval clo

Based on a survey of waste sorting behaviours and perceptions of residents in southern Sweden, we discuss how physical environment, gender and knowledge affect the actual sorting activities.