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A thermostable glycoside hydrolase family-10 xylanase originating from Rhodothermus marinus was cloned and expressed in the methylotrophic yeast Pichia pastoris (SMD1168H). The DNA sequence from Rmxyn10A encoding the xylanase catalytic module was PCR-amplified and cloned in frame with the Saccharomyces cerevisiae alpha-factor secretion signal under the control of the alcohol oxidase (AOX1) promoto
Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co-polymerization of acrylamide with allyl glycidyl ether (AGE) and N,N' metylene-bis(acrylamide) (MBAAm) followed by coupling IDA ligand or (iii) by copolymerization of acrylamide and MBAAm with functional monomer carrying IDA-functionality (1-
We examined the relationship between plant species diversity, productivity and the development of the soil community during early secondary succession on former arable land across Europe. We tested the hypothesis that increasing the initial plant species diversity enhances the biomass production and consequently stimulates soil microbial biomass and abundance of soil invertebrates. We performed fi
The present study argues that political communication on social media is mediated by a platform’s digital architecture—the technical protocols that enable, constrain, and shape user behavior in a virtual space. A framework for understanding digital architectures is introduced, and four platforms (Facebook, Twitter, Instagram, and Snapchat) are compared along the typology. Using the 2016 U.S. elect
Explaining the evolutionary origin and maintenance of color polymorphisms is a major challenge in evolutionary biology. Such polymorphisms are commonly thought to reflect the existence of alternative behavioral or life-history strategies under negative frequency-dependent selection. The European common wall lizard Podarcis muralis exhibits a striking ventral color polymorphism that has been intens
We sequenced multiple genes from the enigmatic genera Bhagadatta Moore, 1898, Kumothales Overlaet, 1940 and Harmilla Aurivillius, 1892 (Nymphalidae, Limenitidinae) and analysed them together with a large published dataset. We find that Bhagadatta is sister to the genera Cymothoe Hübner, 1819+Harma Doubleday, 1848, and that Kumothales is sister to these three. Harmilla is nested within the genus Eu
OBJECTIVE: Previous studies have shown possible neuroendocrine effects of GH. In the present study we investigated the incidence of mental disorders and the prevalence of mental distress and cognitive dysfunction in hypopituitary women with untreated GH deficiency compared to population-based controls.DESIGN AND PATIENTS: Thirty-three hypopituitary women with a median age of 64 years (range 39--77
Stable oxygen and carbon isotope data from a lacustrine sequence at Graenge, southeast Denmark, revealed distinct environmental changes related to Late Weichselian climatic development in the region. Comparison of isotopic records obtained from sedimentary carbonates and freshwater molluscs enabled reconstruction of changes in the lacustrine environment. The degree of thermal and chemical stratifi
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme borreliosis, was estimated by sequence typing of four loci: the rrs-rrlA intergenic spacer (IGS) and the outer-membrane-protein gene p66 on the chromosome, and the outer-membrane-protein genes ospA and ospC on plasmids. The major sources of DNA for PCR amplification and sequencing were samples of the
Studies of the association between circulating IGF-I and cancer risk have shown conflicting results. We have previously observed a U-shaped association between IGF-I and cancer mortality. The present study test the hypotheses of a U-shaped association between IGF-I and incident cancer.
Growth anomaly of trees in some regions was detected under current episode of rapid warming. Thisraises a dilemma for temperature reconstructions by using tree-ring width which is believed to be themost important proxy on inter-annual temperature reconstruction during the past millenniums. Here weemployed the tree-ring d13C to reconstruct temperature variations for exploring their potential oncapt
Measurements of the Standard Model Higgs boson decaying into a bb¯ pair and produced in association with a W or Z boson decaying into leptons, using proton–proton collision data collected between 2015 and 2018 by the ATLAS detector, are presented. The measurements use collisions produced by the Large Hadron Collider at a centre-of-mass energy of s=13Te, corresponding to an integrated luminosity of
The effectiveness of public health measures to prevent COVID-19 contagion has required less vulnerable citizens to pay an individual cost in terms of personal liberty infringement to protect more vulnerable groups. However, the close relationship between scientific experts and politicians in providing information on COVID-19 measures makes it difficult to understand which communication source was
CapsuleHouse Sparrow (Passer domesticus) populations in south Swedish farmland are not affected by supplemental winter feeding, irrespective of agricultural landscape type or presence of animal husbandry, although winter populations declined more in mixed farmland and when farms contained animal husbandry. AimsTo investigate whether food limitation of House Sparrow population size during the winte
Today there are various views on how democracies can be advanced. Bringing participation into decision making processes has been suggested as one method. It is believed that it can give rise to better government, better decisions and better citizens ? but its positive effects will depend heavily on the cultural and local context. Opening up the public sphere for participation is never a simple mat
This study aims to describe how pupils' achievement scores can be affected by the school that the pupil goes to and the pupil's well-being. The participants in this study were upper secondary school pupils. The participants were doing their last year in upper secondary school and they are pupils that have reached the age of 18 years. Questionnaires were used for this study. This method ent
Social movements are an important part of our history. It often reflects conflicts in society, and has many different faces and ambitions. The human rights movement is one of them. Since the 18th century, the thought of human rights have developed and the values of human beings are now protected by laws and conventions. Still to this day, violations of these rights are common. Organizations like A
The international role of the European Union has often been described as the one of a 'normative power', which has the weight to influence the minds and practices of other peoples by its mere attractiveness. However, Human Rights Watch's 2007 World Report concludes; that even if the European Union has the credibility and the power to 'fill the leadership void' in terms of human
