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The X-ray structures of dUTPase from equine infectious anaemia virus (EIAV) in unliganded and complexed forms have been determined to 1.9 and 2.0 A resolution, respectively. The structures were solved by molecular replacement using Escherichia coli dUTPase as search model. The exploitation of a relatively novel refinement approach for the initial model, combining maximum likelihood refinement with

Extensive research is being conducted on magnesium (Mg) alloys for bone implant manufacturing, due to their biocompatibility, biodegradability and mechanical properties. Gadolinium (Gd) is among the most promising alloying elements for property control in Mg alloy implants; however, its toxicity is controversial. Investigating Gd behavior during implant corrosion is thus of utmost importance. In t

Pentaklorfenol (PCP) är en klorerad aromatisk förening som användes flitigt för att behandla trä mot mikrobiell nedbrytning innan den blev förbjuden på 70-talet. På grund av dess höga löslighet förekommer PCP som en grundvattenförorening kring äldre sågverk. Hjortsberga i södra Sverige är ett exempel på denna typ av förorening och är det område som undersöks i arbetet. Förorenat grundvatten spridsPentachlorophenol (PCP) is a chlorinated aromatic compound that was commonly used for treatment of wood against microbial degradation before it got banned in the 1970s. Due to its solubility in water, PCP is a common groundwater contaminant around old sawmills, for example near Hjortsberga in southern Sweden where this study was performed.. The contaminated groundwater is spreading and leaking int

The global nature of businesses has increased the geographical dispersion of operations and has made the location decision an important strategic perspective for manufacturing firms. The consensus view is that three major plant location factors must be kept in mind: (1) access to low-cost manufacturing, (2) proximity to market, and (3) access to development competences. Two options for changing th

M1 aminopeptidases comprise a large family of biologically important zinc enzymes. We show that peptide turnover by the M1 prototype, leukotriene A4 hydrolase/aminopeptidase, involves a shift in substrate position associated with exchange of zinc coordinating groups, while maintaining the overall coordination geometry. The transition state is stabilized by residues conserved among M1 members and i

Wildfires are a natural disturbance shaping boreal forests, but their effects on soil microbial communities and biogeochemical cycling remain unclear, particularly in Fennoscandia. We investigated the impact of fire severity and post-fire management on the soil microbial community two years after a boreal wildfire in Sweden. We quantified growth rates of bacteria, saprotrophic fungi (SF), ectomyco

In the past two decades, the eddy covariance technique has been used for an increasing number of methane flux studies at an ecosystem scale. Previously, most of these studies used a closed path setup with a tunable diode laser spectrometer (TDL). Although this method worked well, the TDL has to be calibrated regularly and cooled with liquid nitrogen or a cryogenic system, which limits its use in r

Effects of hydrogen bonding and the axial ligand interaction on the B800 band in two LH2 complexes Rhodopseudomonas (Rps.) acidophila and Rhodospirillum (Rs.) molischianum have been theo retically investigated by using density functional theory. The local electrostatic environment of the B800 bacteriochlorophyll is simulated as an atomic charge field consisting of the pigments in the protomer unit

The Amazon Basin is a unique region to study atmospheric aerosols, given their relevance for the regional hydrological cycle and the large uncertainty of their sources. Multi-year datasets are crucial when contrasting periods of natural conditions and periods influenced by anthropogenic emissions. In the wet season, biogenic sources and processes prevail, and the Amazonian atmospheric composition

Chemical communication by means of sex pheromones is central to the mating systems of a wide range of organisms. Because reproductive isolation is often based on pheromone differences, understanding how pheromones diverge is necessary for a complete comprehension of the speciation process. Moths (Insecta: Lepidoptera) contributes highly relevant examples to the theory of speciation because subtle

Lund University conducts active fundraising to increase philanthropic funding for the university's activities. Target groups are private foundations, companies, organizations and individuals. The Development Office is the central hub for generating these types of donations and gifts. Our overall goal with the operation is that Lund University and our donors, in their own unique way, should be able

The Sound Environment Centre at Lund University was established in March 2005. The centre is the first of its kind, both nationally and internationally. It is tasked with studying sound as a phenomenon, sound worlds and sound environments from a multi- and interdisciplinary perspective. It is a Lund University-wide body for education and research as well as external engagement in general for matte

Bakteriella infektioner är ett stort globalt hälsoproblem och mer detaljerad kunskap om värd-bakterieinteraktioner är nödvändiga för att kunna bemöta den ökande antibiotikaresistensen. Nordenfelt-labbet använder unik biosensorteknologi tillsammans med avancerad videomikroskopi och bildanalystekniker för att studera tidiga värd-bakterieinteraktioner. Den molekylära sammansättningen av bakterier ochBacterial infections represent a major global health issue and more detailed knowledge about host-bacteria interactions are needed to counteract the rising bacterial resistance to antibiotics. The Nordenfelt Lab uses unique biosensor technology in conjunction with advanced live imaging and image analysis techniques to study initial host-pathogen interactions. The molecular surface composition of

-Reduced arterial compliance precedes changes in blood pressure, which may be mediated through alterations in vessel wall matrix composition. We investigated the effect of the collagen type I-alpha1 gene (COL1A1) +2046G>T polymorphism on arterial compliance in healthy individuals. We recruited 489 subjects (251 men and 238 women; mean age, 22.6+/-1.6 years). COL1A1 genotypes were determined using

Ligand induced activation of the beta-receptor for platelet-derived growth factor (PDGF) leads to activation of Src family tyrosine kinases. We have explored the possibility that the receptor itself is a substrate for Src. We show that Tyr934 in the kinase domain of the PDGF receptor is phosphorylated by Src. Cell lines expressing a beta-receptor mutant, in which Tyr934 was replaced with a phenyal