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A simple labeling approach is presented based on protein expression in [1-C-13]- or [2-C-13]-glucose containing media that produces molecules enriched at methyl carbon positions or backbone C-alpha sites, respectively. All of the methyl groups, with the exception of Thr and Ile(delta 1) are produced with isolated C-13 spins (i.e., no C-13-C-13 one bond couplings), facilitating studies of dynamics

A series of copolymers of N-isopropylacrylamide (NIPAM) and the more hydrophobic comonomer N-tert-butylacrylamide (NTBAM), with increasing NTBAM content (i.e., increasing hydrophobicity) were prepared. The adhesion of human epithelial cells on polymer films prepared from copolymers of NIPAM: NTBAM was observed to increase with increasing polymer hydrophobicity. However, in the absence of serum, ce

Long-term records of environmental history at decadal to millennial time-scales enable an assessment of ecosystem variability and responses to past anthropogenic disturbances and are fundamental for the development of environmental management strategies. This study examines the local variability of land-use history in the South Swedish Uplands over the last 200 years based on pollen records from t

Intraluminal pressure has a significant impact on vascular adaptability, phenotype and regulation of blood flow and pressure. On one hand, increased pressure/stretch for a prolonged time can cause structural changes in vessel wall; on the other hand, lack of pressure/stretch can promote a phenotype shift. This thesis investigates novel roles of microRNAs and protein tyrosine kinase 2 in pressure/s

An invertebrate nerve cell preparation, the lobster stretch receptor neuron, was investigated with respect to maintenance of its resting voltage, intracellular ion concentrations and excitability using voltage clamp technique and ion-selective microelectrodes. It was found that the resting voltage (-65 mV) of microelectrode impaled cells is caused by (a) a homogenous population of K+ channels whic

Grass pea (Lathyrus sativus) is a leguminous food crop, grown mainly in the tropical and subtropical regions. It has valuable nutrients, however consumption of the seeds is reported to cause lathyrism, due to the presence of a non-protein amino acid (?-ODAP). The present study deals with the development of a process for reducing the ?-ODAP content in grass pea seeds. The consequences of processin

Popular Abstract in Swedish I följande arbete har ett dynamiskt modellbibliotek av komponenter utvecklats för processlinjer. Analysen inriktades mot karakteristiska aspekter för processlinjer för flytande livsmedel, och nya modeller utvecklades för byte av fluidum, dispersion, blandfaser och första ordningens kemiska reaktioner i rör och värmeväxlare. Modellernas beräkningseffektivitet och noggranIn the present work a dynamic model library of components for process lines was developed. The analysis addressed characteristic aspects of liquid food process lines, and new models were developed for fluid transitions, dispersion, mixing zones and first-order chemical reactions in pipes and heat exchangers. The computational efficiency and accuracy of the models were analysed. It was demonstrated

Methods for sampling and determination of four organic acid anhydrides are described. The methods were validated in a standard atmosphere and in the work environment. Hexahydrophthalic anhydride (HHPA), methylhexahydrophthalic anhydride (MHHPA), tetrahydrophthalic anhydride (THPA) and octenylsuccinic anhydride (OSA) were sampled using Tenax and Amberlite XAD-2 solid sorbents and determined by GC w

The role of workplace supervisors in the clinical education of medical students is currently under debate. However, few studies have addressed how supervisors conceptualize workplace learning and how conceptions relate to current sociocultural workplace learning theory. We explored physician conceptions of: (a) medical student learning in the clinical workplace and (b) how they contribute to stude

Prospective cohort studies about cutaneous melanoma (CM) risk are still few. Host factor- and UVR exposure data were collected prospectively by questionnaire in this population-based cohort study including 40000 Swedish born women, aged 25-64 years at enrolment (1990). Risk for CM (Cox regression and Stepwise Cox regression [SCR], hazard ratios [HRs] with 95 % Confidence Intervals [CI]) in relatio

The t (t) over bar production cross-section dependence on jet multiplicity and jet transverse momentum is reported for proton-proton collisions at a centre-of-mass energy of 7 TeV in the single-lepton channel. The data were collected with the ATLAS detector at the CERN Large Hadron Collider and comprise the full 2011 data sample corresponding to an integrated luminosity of 4.6 fb(-1). Differential

In hermaphroditic plants, the effect of a social environment on sex allocation has not been studied to our knowledge, while in hermaphroditic animals such effects are known to be common. In recent years, studies on root competition beyond the effects of nutrients have shown that plants can respond to their conspecific root competitors (social environment), making it interesting to ask if these eff

P>The flux of carbon from tree photosynthesis through roots to ectomycorrhizal (ECM) fungi and other soil organisms is assumed to vary with season and with edaphic factors such as nitrogen availability, but these effects have not been quantified directly in the field. To address this deficiency, we conducted high temporal-resolution tracing of 13C from canopy photosynthesis to different groups of

Background: Stroke in the young has not been thoroughly investigated with most previous studies based on a small number of patients from single centers. Furthermore, recent reports indicate that Fabry disease may be a significant cause for young stroke. The primary aim of our study was to determine the prevalence of Fabry disease in young stroke patients, while the secondary aim was to describe pa

This article provides 12-month prevalence rates of youth exposure to websites which encourage self-harm or suicide and examines whether such exposure is related to thoughts of self-harm and thoughts of suicide in the past 30 days. Data were collected via telephone from a nationally representative survey of 1560 Internet-using youth, ages 10-17 residing in the United States. One percent (95% CI: 0.

Objectives: To verify the diagnostic accuracy of anti-double-stranded DNA (anti-dsDNA) antibodies detected by the Crithidia luciliae immunofluorescence test (CLIFT) in a cohort of unselected patients, referred to a rheumatologist due to recent onset of rheumatic symptoms. Method: A total of 1073 consecutive patients were screened for anti-nuclear antibodies (ANAs). Serum samples from 292 ANA-posit