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Oxygen supply is a critical point in technical processes when aerobic cells are used in immobilized preparations. This report concerns the use of hemoglobin or emulsions of perfluorochemicals (completely fluorinated organic compounds) to carry oxygen to immobilized cells. Both methods work well and do not seem to harm the cells. The perfluorochemical method of improving oxygen supply showed a high

In this study we investigated receptor-specific cellular signals elicited by kinin agonists in cultured rabbit superior mesenteric artery smooth muscle cells. Kinins promoted an increase in inositol phosphate formation and arachidonic acid release in these cells. The responses elicited by des- Arg9-bradykinin (des-Arg9-BK), a B1 kinin agonist, were antagonized by des- Arg9[Leu8]-BK, a B1 kinin ant

Bradykinin (BK) and bombesin (BN) stimulate an increase in the tyrosine phosphorylation of a 120-kDa group of proteins (pps120) in Swiss 3T3 cells (Leeb-Lundberg, L. M. F., and Song X.-H. (1991) J. Biol. Chem. 266, 7746-7749). Here, we show that a component of pps120, p125, was specifically immunoprecipitated with antibodies against the p21ras GTPase-activating protein (GAP). The major portion of

Bradykinin receptors have been identified in human gingival fibroblasts; the primary signal transduction pathways and their dependence on calcium have been characterized. Binding data revealed a calcium-independent binding of bradykinin to the cell membrane with a receptor density of 25,000 receptors per cell and a K d of 1.6 nM. The bradykinin receptor-mediated activation of phospholipase C (PLC

This study was undertaken to evaluate the role of internalization in the action of the peptide autacoid bradykinin (BK). At 4°C [3H]BK binds to an apparently single class of B2 kinin receptors on DDT1 MF-2 smooth muscle cells (C. M. Munoz, S. Cotecchia, and L. M. F. Leeb-Lundberg, manuscript submitted). At this temperature the [3H]BK binding was confined exclusively to the cell surface. On the oth

We have examined the effect of bradykinin (BK) and other peptide mediators with related cellular actions on tyrosine phosphorylation in confluent Swiss 3T3 fibroblast cells using an anti-phosphotyrosine antibody. Immunoblots of extracts from cells stimulated with BK showed a major heterogeneous band centered at Mr 120,000. Three phosphorylated protein species were present within this band. The low

The two pyrazolopyridines, etazolate (SQ20009) and cartazolate (SQ65396), enhance the binding of [3H]diazepam to benzodiazepine receptor sites in rat brain. This enhancement is due to a change in affinity without a change in maximal binding. Pentobarbital also enhances [3H]diazepam binding by lowering the K(D). Pentobarbital gives a maximal enhancement of benzodiazepine binding slightly greater th

Maps of the MT-saturation as derived in FLASH-based multi-parameter mapping (MPM) are largely corrected for effects of flip angle inhomogeneity. Here, we present a heuristic model for residual effects of B1+ that is rooted in the dynamics of macromolecule saturation. Model parameters were validated for brain tissues by variation of the MT-pulse flip angle in an established MPM protocol. White and

This study addresses gender assignment in six North Scandinavian varieties with a three-gender system: Old Norse, Norwegian (Nynorsk), Old Swedish, Nysvenska, Jamtlandic, and Elfdalian. Focusing on gender variation and change, we investigate the role of various factors in gender change. Using the contemporary Swedish varieties Jamtlandic and Elfdalian as a basis, we compare gender assignment in ot

Wide-angle neutron scattering experiments combined with empirical potential structural refinement modeling have been used to study the detailed structure of decyltrimethylammonium bromide micelles in the presence of acid solutions of HCl or HBr. These experiments demonstrate considerable variation in micelle structure and water structuring between micelles in the two acid solutions and in comparis

The paper investigates the status of general meanings in prepositional semantics. On the basis of a review of the strategies employed in different accounts of prepositions, the paper argues that strict adherence to the notion of general meanings is unfeasible in the field, partly because a purely deductive method of description breaks down as the number of possible oppositions increases. This fail

The rising price and demand for energy are significant issues for the food sector, which consumes a substantial amount of energy throughout the supply chain. Hence, improving energy efficiency has become an essential priority for the food sector. However, most food businesses have limited awareness of the recent technological advancements in real-time energy monitoring. Thus, the concept of “Inter

Despite the growth in scholarship on diverse religious communities in Turkey, little attention has been paid to Twelver Shiʿi Muslims. Since the founding of the Republic, the Turkish state's foundational secularist agenda has attempted to control and promote a single hegemonic form of Islam, and Shiʿa have faced continuous issues practicing their faith in public as a result. While the liberalizati

Saltwater intrusion is a serious environmental problem in coastal subsurface water systems around the world. In the development of subsurface water protection and rehabilitation strategies in the coastal areas, mathematical models play an important role. This paper discusses the role of subsurface water contamination models in planning, management and regulation, with a focus on generic and site s

About 40 years ago, a subpopulation of small neurons in the cerebellar nuclei was shown to project to the inferior olive, the source of the climbing fiber input to the cerebellum. This nucleo-olivary projection follows the zonal and probably also microzonal arrangement of the cerebellum so that closed loops are formed between the neurons in the olive, the cerebellar cortex, and the nuclei. Althoug

The rewriting of hagiographical texts was a common activity in Byzantium. From at least the ninth century until the end of the Byzantine era, ecclesiastical and non-ecclesiastical writers turned repeatedly to this form of writing, in some cases evidencing an astonishingly prolific textual output. Metaphrasis is the only word found in Byzantine titles to indicate a rewritten text but never used to

Objective: To describe maternal perception of the quality of maternal and newborn care (QMNC) in facilities in Norway during the first year of COVID-19 pandemic. Methods: Women who gave birth in a Norwegian facility from March 1, 2020, to October 28, 2021, filled out a structured online questionnaire based on 40 WHO standards-based quality measures. Quantile regression analysis was performed to as