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Escherichia coli is a microorganism routinely used in the production of heterologous proteins. The overexpression of a xylanase (Xyn10A Delta NC), which originated from the thermophile Rhodo-thermus marinus cloned under the control of the strong T7/lac promoter in a defined medium (mAT) using a substrate-limited feed strategy, was however shown to impose a significant metabolic burden on host cell

Process parameter optimization for the supercritical CO2 extraction of rye bran to obtain alkylresorcinols (AR) was studied by carrying out a two-level fractional design experiment. Four parameters, temperature, CO2 flow rate, cosolvent percentage, and extraction time, presumed to influence the extraction process, were analyzed. A tentative fractionation of the crude extract was also carried out a

BACKGROUND: Consumption of soluble dietary fibre is correlated with decreased postprandial glucose and insulin responses and hence has beneficial effects on the metabolic syndrome. AIM OF THE STUDY: To investigate the effects on postprandial glucose, insulin and triglyceride concentrations of meals enriched with soluble dietary fibres from oats, rye bran, sugar beet fibre or a mixture of these thr

A set of 165 strains of different staphylococcal species, 67 Staphylococcus aureus, 71 novobiocin-sensitive coagulase-negative staphylococci (CNS) and 27 novobiocin-resistant CNS was used. The oxacillin and methicillin MICs were recorded after 24 and 42 h of incubation at 35 degrees C and at 30 degrees C. Significantly higher MICs were recorded at 30 degrees C compared with 35 degrees C. While a p

We used a high-titer recombinant adeno-associated virus (rAAV) vector to express WT or mutant human alpha -synuclein in the substantia nigra of adult marmosets. The alpha -synuclein protein was expressed in 90-95% of all nigral dopamine neurons and distributed by anterograde transport throughout their axonal and dendritic projections. The transduced neurons developed severe neuronal pathology, inc

The present study focuses on the characteristics of subject-verb agreement in number in spoken and written French, two morphological systems that are very different. In particular, we investigate the impact of frequency of forms and morphological patterns in the input on the acquisition of number agreement in monolingual and bilingual French-speaking children (n=32). Our results demonstrate an alm

In this article I focus on some of Joseph Margolis's contributions to medical ethics. I first discuss some of Margolis's normative and metaphysical views on death and abortion, particularly in his early work Negativities, as well as some of his metaphysical assumptions. Then these views and assumptions are related to his theory of persons and, by implication, his theory of culture, set forth in a

Chloroplasts isolated from a plant species, Dimorphotheca pluvialis Moench (Asteraceae), grown under visible light without supplementary UV-B radiation, have poor grana development. The dominant components in the membrane organizations are doublets and triplets of stroma lamellae. However, such chloroplasts possess the capacity for electron transfer through the whole photosynthetic chain, based on

1. Task-dependent modulation of cutaneous input to climbing fibres projecting to the C1, C2 and C3 zones in the cerebellar paravermal lobule V was investigated in awake cats during performance of a reaching task. 2. Climbing fibre responses resulting from low intensity (non-noxious) electrical stimulation of the ipsilateral superficial radial nerve were recorded as extracellular field potentials i

The aim of this project was to investigate possible methods for the concentration of acid hydrolyzate obtained from hydrolyzing wood. A detailed study of concentration of sugar solution by reverse osmosis, RO, has been performed as well as a comparison with mechanical vapor recompression evaporation. The comparison has, in this specific case, shown that RO is currently more expensive. The result i

The first acedemic volume on the history, ideology and social history of Swedish freemasonry. Contains a membership list with 4300 members in Swedish lodges of the 18th century.

Previous results have shown that severe, prolonged hypoglycemia leads to neuronal cell damage in, among other structures, the cerebral cortex and the hippocampus but not the cerebellum. In order to study whether or not this sparing of cerebellar cells is due to preservation of cerebellar energy stores, hypoglycemia of sufficient severity to abolish spontaneous EEG activity was induced for 30 and 6

Serotonin (5-HT) at low concentrations induces an endothelium-dependent relaxation in the rat jugular vein mediated via a 5-HT1-like receptor. The receptor mediating this relaxation was characterized in vitro using agonists and antagonists in segments precontracted with prostaglandin F2 alpha in the presence of the 5-HT2 receptor antagonist ketanserin. The following substances acted as agonists, w

Using laser-Doppler flowmetry during ventriculocisternal perfusion with inulin-[14C]carboxylic acid, choroid plexus blood flow (CPBF) and CSF production were measured simultaneously in rats during periods of 3 h. Blood flow and CSF production decreased only slightly during control experiments. The effect of vasoactive intestinal polypeptide (VIP) was studied at different concentrations of the pept