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Here, we present a workflow for transcriptional silencing of transposable elements (TEs) in human induced pluripotent stem cells (hiPSCs). We describe steps for designing guide RNAs (gRNAs) to target TE families or unique TE loci. We also detail procedures for validating the efficiency and specificity of large-scale CRISPRi-based silencing using a multiome approach combining bulk RNA sequencing, C
Background: Environmental enrichment (EE) has been widely reported to improve functional recovery after stroke, yet its effects on chronic inflammation and white matter pathology remain less well defined. The aim of this study was to investigate whether poststroke EE modulates chronic neuroinflammation and white matter pathology and how these changes relate to functional recovery. Methods: Here, w
Randomized controlled trials (RCTs) of fecal microbiota transplantation (FMT) in patients with irritable bowel syndrome (IBS) have produced outcomes varying from no effect at all to high efficacy and durable effects over time. This review analyzed differences in the protocols used in FMT RCTs for IBS in the recently published literature with the aim of identifying the factors responsible for the s
BACKGROUND: Identifying the underlying cause of acute pancreatitis is fundamental for prevention of recurrence. However, no standardised algorithm for determining its aetiology exists.AIMS: This survey study aimed to investigate the international variations in diagnosis, workup protocols and follow-up of acute pancreatitis.METHODS: A 37-item international questionnaire was developed by a multidisc
During an infection, the genetic composition of the pathogen population in the host might range from clonal to genetically diverse, depending on the genetic variation of the inoculum, mutation events, genetic drift, and host selection during the course of the infection. Variation in the genetic diversity of avian malaria parasites, both within and between individual hosts, is not well documented.
Boreal and temperate forests have been greatly affected by clear-cutting. Alternative management systems, such as continuous cover forestry and selective logging, have remained secondary to the dominant even-aged management regime. This regime has its roots in scientific forest management, whose dissemination and development have been well described. But the experiences of rural communities living
Cu-based ternary catalysts often outperform their binary counterparts in the hydrogenation of CO2 to methanol. Unraveling the underlying synergistic effects among multiple components remains challenging and requires comprehensive operando characterization. In this study, we present a detailed investigation into the synergistic Cu−Zn−Zr interactions in inverse ZnZrOx/Cu catalysts, which show strong
AI is transforming how students learn, raising concerns about whether it expands educational opportunities or widens existing gaps. We examine this question in a preregistered lab experiment (N=572) in which students study a novel topic under one of three conditions: browsing only (control), AI-assisted, or AI-guided, and then complete an exam without AI access. We find no overall effect of AI acc
Artificial Intelligence is advancing rapidly, raising critical concerns about the integrity of digital content, particularly in sensitive domains such as medical imaging. Recent AI techniques, such as Generative Adversarial Networks (GANs) and diffusion models, can generate highly realistic synthetic medical images, posing risks of misdiagnosis, inappropriate treatment, and other adverse outcomes.
We explore configurations of sufficient and necessary conditions for the evolution and resilience of borrower trust in their lenders. Because trust is a dynamic phenomenon that needs to be understood in terms of change over time, we rely on longitudinal data collected from managers of small- and medium-sized enterprises (SME) who are clients of cooperative banks operating in Austria and Finland, a
Explicit solutions to optimal control problems are rarely obtainable. Of particular interest are the explicit solutions derived for minimax problems, providing a frame work to address adversarial conditions and uncertainty. This work considers a multi-disturbance minimax Linear Regulator (LR) framework for positive linear time-invariant systems in continuous time, which, analogous to the Linear-Qu
Various membrane-based extraction techniques are used for sample pretreatment in analytical chemistry prior to using chromatographic or other techniques for the final determination. Such techniques show several advantages over other extraction. They lead to large selectively for several types of analytes in even complex samples and high-concentration enrichment, easily thousands of times. The most
This chapter sets the stage for the volume. Sustainable consumption has long been on the global agenda, yet prevailing consumption patterns continue to drive climate change, resource depletion and biodiversity loss. One of the key barriers is the persistence of myths – narratives that render unsustainable practices normal, desirable or even virtuous. The chapter situates myths historically, tracin
Background: National studies on primary malignant tumours arising from the external auditory canal (EAC) are rare. Objectives: To investigate the incidence and outcomes of squamous cell carcinoma of the external auditory canal (SCC-EAC) in Sweden. Materials and methods: The retrospective study included patients diagnosed with SCC-EAC in Sweden from 2007 to 2017. Results: Forty-six patients with SC
Feedbacks from the climate to other components of the coupled human-Earth system are expected to strongly influence the co-evolution of human society and its environment. Representing these feedback loops between climate and society, via the Earth system's response to human activities and the subsequent effect back onto social systems, is essential in order to fully explore the dynamics of the cou
The chaperone DNAJB6 inhibits aggregation of several amyloid proteins, such as α-synuclein and huntingtin, which are involved in neurodegenerative diseases. Here, we designed a cell-based assay to measure DNAJB6 dimerization in HEK293 cells by fluorescent resonance energy transfer (FRET), as previous studies suggest that the activity of DNAJB6 is dependent on dimerization. The HEK293 cells were en
Gliomas that express the mutated isoforms of isocitrate dehydrogenase 1/2 (IDH1/2) have better prognosis than wild-type (wt) IDH1/2 gliomas. However, how these mutant (mut) proteins affect the tumor microenvironment is still a pending question. Here, we describe that the transcription of microtubule-associated protein TAU (MAPT), a gene that has been classically associated with neurodegenerative d
