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Compatible solutes are small, soluble organic compounds that have the ability to stabilise proteins against various stress conditions. In this study, the protective effect of ectoines against pH stress is examined using a recombinant xylanase from Bacillus halodurans as a model. Ectoines improved the enzyme stability at low (4.5 and 5.0) and high pH (11 and 12); stabilisation effect of hydroxyecto
Although it is generally agreed that the Arctic flora is among the youngest and least diverse on Earth, the processes that shaped it are poorly understood. Here we present 50 thousand years (kyr) of Arctic vegetation history, derived from the first large-scale ancient DNA metabarcoding study of circumpolar plant diversity. For this interval we also explore nematode diversity as a proxy for modelli
Functionalities of clay-polymer nanocomposites (CPN) are related to the degree of clay particle exfoliation and orientation within the polymer matrix. Exploration of new physical methods for such CPN processing is currently an active field of research. In the present work, organoclay polypropylene nanocomposites were prepared by melt intercalation and subsequently exposed to an electric field (E)
Objectives: To examine the risk of putative pneumococcal infections in adult arthritis patients on different anti-rheumatic drugs immunized with heptavalent pneumococcal conjugate vaccine (Prevenar 7; PCV7) and non-vaccinated individually matched arthritis patients. Method: All individuals in a cohort of 505 patients with rheumatoid arthritis (RA) or spondylarthropathy (SpA) receiving different an
Nasal decolonisation has a proven effect on the prevention of severe Staphylococcus aureus infections and in the control of methicillin resistant S. aureus (MRSA). However, rising rates of resistance to antibiotics highlights the need for new substances for nasal decolonisation. LTX-109 is a broad spectrum, fast-acting bactericidal antimicrobial drug for topical treatment, which causes membrane di
The Bushveld Complex in South Africa represents the world's largest intrusion of magnesium-and iron-rich magmas. The Bushveld magmas were emplaced beneath the Transvaal basin(1) similar to 2.06 billion years ago(2,3), but their origin remains elusive. The magmas may have formed in response to an upwelling mantle plume(4), ancient subduction5 or melting triggered by a meteorite impact(6). Here we u
We demonstrate a method that enables the study of photoluminescence of as-grown nanowires on a native substrate by non-destructively suppressing the contribution of substrate photoluminescence. This is achieved by using polarized photo-excitation and photoluminescence and by making an appropriate choice of incident angle of both excitation beam and photoluminescence collection direction. Using T-p
Nanoparticles made from recombinant proteins offer excellent potential for several nanotechnological applications. However, only a very limited number of proteins are currently being used for such purposes due to limited availability and stability. Therefore, we have investigated the enamel matrix protein amelogenin as a new alternative protein for use as recombinant nanoparticles. Amelogenin is a
Background: Elemental mercury (Hg0) is widely used in small-scale gold mining. Individuals working or living in mining areas have high urinary concentrations of Hg (U-Hg). Differences in genes encoding potential Hg-transporters may affect uptake and elimination of Hg. Objective: To identify single nucleotide polymorphisms (SNPs) in Hg-transporter genes that modify U-Hg. Methods: 1,017 men and wome
Protein and peptide self-assembly is a powerful design principle for engineering of new biomolecules. More sophisticated biomaterials could be built if both the structure of the overall assembly and that of the self-assembling building block could be controlled. To approach this problem, we developed a computational design protocol to enable de novo design of self-assembling peptides with predefin
The aim was to study long-term effects on motor skills and school performance of increased physical education (PE). All pupils born 1990-1992 from one school were included in a longitudinal study over nine years. An intervention group (n = 129) achieved daily PE (5 × 45 min/week) and if needed one extra lesson of adapted motor training. The control group (n = 91) had PE two lessons/week. Motor ski
Photosynthesis is highly responsive to environmental and physiological variables, including phenology, foliage nitrogen (N) content, atmospheric CO2 concentration ([CO2]), irradiation (Q), air temperature (T) and vapour pressure deficit (D). Each of these responses is likely to be modified by long-term changes in climatic conditions such as rising air temperature and [CO2]. When modelling photosyn
InAs nanowires have been used to fabricate high-performance MOSFETs that have been integrated into small-scale RF-circuits. We describe the strategy for the design of the transistor architecture and present data for the DC and high-frequency performance. Studies of the 1/f-noise show competitive normalized noise spectral density although it suggests the presence of defects within the high-k film t
Advances in next-generation RNA-sequencing have revealed the complexity of transcriptomes by allowing both coding and noncoding (nc) RNAs to be analyzed. However, limited data exist regarding the whole transcriptional landscape of chronic lymphocytic leukemia (CLL). In this pilot-study, we evaluated RNA-sequencing in CLL by comparing two subsets which carry almost identical or `` stereotyped'' B-c
Unsteady wind loads due to turbulence within the telescope enclosure are one of the largest dynamic disturbances for ground-based optical telescopes. The desire to minimize the response to the wind influences the design of the telescope enclosure, structure, and control systems. There is now significant experience in detailed integrated modeling to predict image jitter due to wind. Based on this e
Red blood cell distribution width (RDW), a measure of variation in erythrocyte volume, has been associated with several cardiovascular disorders, but the relationship with atrial fibrillation (AF) remains unclear. We investigated the association between RDW and incidence of first hospitalization due to AF in a population-based cohort.
A crucial stage in the interaction between pollinators and plants is the moment of physical contact between them, known as flower inspection, or handling. Floral guides - conspicuous colour markings, or structural features of flower corollas - have been shown to be important in the inspecting behaviour of many insects, particularly in diurnal species. For the nocturnal hawkmoth Manduca sexta tacti
The rate of spontaneous pregnancies in women with Turner syndrome (TS) is low (2% to 5%). Oocyte donation is an option for these women and enables many to become pregnant. Some investigators question the use of pregnancy induction in TS because of the high risk for aortic dissection or other serious cardiac events. A cardiac evaluation is recommended before pregnancy is planned in TS. Among patien
