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In this study, NaCl pellets read with optically stimulated luminescence were evaluated for their potential use as a point dosemeter in breast x-ray imaging. Dosimetry with NaCl pellets has previously been applied to various environmental and medical settings. NaCl pellets have potential in clinical breast dosimetry because they can enable multiple point measurements to be simultaneously conducted
Negative consequences of gambling problems have primarily been examined in terms of symptoms and impairment, with less focus on well-being, a key indicator of intra- and interpersonal functioning and a critical outcome in treatment. Additionally, the role of co-occurring psychopathology in this relation remains unclear. This study examined the relation between gambling problems and well-being in a
The objectives of this study were to explore active aging among people listed with an interest in relocation, and the relationships between active aging and aspects of perceived home. Using cross-sectional data from the 2022 RELOC-AGE project ( N = 1,509, mean age = 70 years), linear regression analysis was conducted. Women and individuals with higher self-rated health and education reported hig
Background: Gaming disorder has recently been recognized as a psychiatric condition, yet the clinical characteristics of treatment-seeking individuals remain understudied. This study examined youth and adults seeking treatment at a specialized outpatient clinic in southern Sweden.Methods: A total of 107 individuals aged 12-49 years (M = 22.1, SD = 7.2) underwent comprehensive clinical interviews,
BackgroundSymptom severity cutoffs for body dysmorphic disorder (BDD) are lacking, hindering communication between professionals and with the patient community.MethodWe pooled data from 11 clinical trials or high-quality cohort studies from specialist clinics, totaling 804 individuals with BDD (80 % girls/women, 67 % adults). All participants had baseline scores on the adult or adolescent versions
Reliable assessment of self-reported immune function is essential for identifying at-risk populations, monitoring public health interventions and informing global health strategies. Yet, current instruments are imprecise and lack cross-cultural validity. This study aimed to assess the cross-cultural validity of the 7-item Immune Status Questionnaire (ISQ). The Partial Credit Rasch model was applie
A large number of cities have created semantic 3D city models, but these models are rarely used as input data for simulations, such as noise and flooding, in the urban planning process. Reasons for this are that many simulations require detailed land cover (LC) and elevation data that are often not included in the 3D city models, and that there is no linkage between the elevation and land cover da
Children and adolescents with obsessive-compulsive disorder (OCD) are at increased risk of psychotic disorders; however, the manifestations and clinical implications of psychotic vulnerability among youth with OCD remain poorly understood. To address this gap, we examined the prevalence and clinical correlates of psychotic vulnerability in youth with OCD across two independent samples. Using the T
Posttraumatic stress disorder (PTSD) and obsessive-compulsive disorder (OCD) share ostensibly similar and sometimes overlapping symptoms that complicate diagnostic assessment and conceptualization. While varying models for the symptom structure of PTSD have been proposed – including the presently used 4-factor and a more fine-grained 7-factor model – little research has focused on youth and even l
This study investigates the integration of Large Language Models with the TRIZ to improve problem solving and innovation in industrial product development. By combining the structured problem-solving framework of TRIZ with LLMs to process large amounts of data and generate ideas, this hybrid approach seeks to overcome the limitations of traditional TRIZ and optimize solution generation. In a case
Water is most important to all lives on this planet, but there is a lot of fresh water wasted on the land. With the rapid increase of population, the humankind shall pay more attention to water storage on the land. Inspired by the “sponge city” theory put forward by some researchers in urban construction in recent years, the authors try to put forward the “sponge earth” theory, so as to make enoug
As universities compete for higher standings in global rankings and implement performance management measures, performance pressure is imposed on scholars. This paper explores the influence of academic systems and career models on scholars in Sweden and Finland, with a focus on experienced performance pressure. Comparable qualitative and quantitative data from these Nordic countries were collected
OBJECTIVE: To explore the cerebrospinal fluid (CSF) proteome in systemic lupus erythematosus (SLE) and the associations between the CSF proteomic patterns and clinical manifestations.METHODS: CSF samples from 29 female outpatients with SLE were analyzed with label-free liquid chromatography tandem mass spectrometry. Inclusion and CSF collection were conducted irrespective of clinical manifestation
Portal hypertension (PH) is a major complication of chronic liver disease, often leading to serious clinical consequences such as variceal bleeding, ascites, and splenomegaly. The current gold standard for PH diagnosis, namely, hepatic venous pressure gradient measurement, is invasive and not widely available. Transient elastography has emerged as a non-invasive alternative for assessing liver sti
Hydroxylamine was used to cleave the Asn-Gly peptide bond between the fusion partner and the antimicrobial peptide of interest, a magainin derivative (MSI-344). The efficiency of reaction depended on the hydroxylamine concentration, denaturant, pH, and the fused protein concentration. The optimal cleavage solution consisted of guanidine ⋅ HCl as the denaturant, pH 8.1, and 6.7 mg ml−1 of fuse
Triton X-114 and cation-exchange chromatography, SP-Sepharose FF, removed endotoxins from solutions containing recombinant histone H1.5. Dissociated endotoxins were removed but fractions containing histone H1.5 were enhanced in the elution step. The final concentration of endotoxins, measured by a limulus amoebocyte lysate (LAL) assay, was below 0.05 EU mg−1 histone H1.5. The recovery of protein w
A magainin derivative, designated MSI-344, was produced in Escherichia coli as fusion protein, by utilizing a truncated amidophsphoribosyltransferase of E. coli as a fusion partner. Bacterial cells transformed with the gene encoding the fusion protein were grown to a high cell density and induced with isopropyl-1-thio-b-D-galatoside (IPTG) to initiate product expression. The fusion protein was
An Escherichia coli expression system has been constructed for production of biologically active recombinant histone H1.5. A process of fermentation and purification method at a large scale has been developed. Recombinant histone H1.5 was released from the high density cultured cells by high-pressure homogenization. For an efficient removal of cell debris and partial purification of basic histone
An Escherichia coli expression system was constructed for production of a recombinant antimicrobial peptide buforin derivative named buforin IIb and a large-scale process of cultivation and purification was also developed. Inclusion bodies of fusion proteins containing recombinant buforin IIb were released from high-density-cultured cells by high-pressure homogenization. The inclusion bodies
Paclitaxel was purified from cell cultures of Taxus chinensis by a combination of extraction, low-pressure chromatography, precipitation and HPLC. A crude extract (purity 6.9%) was obtained by methanol extraction of plant cell cultures, followed by liquid–liquid extraction using dichloromethane. The extract was purified to greater than 32% with a 97% step yield by low-pressure chromatography.
