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Embryonic ventral mesencephalic tissue from the pig is a potential alternative donor tissue for neural transplantation to Parkinson's disease patients. For stable graft survival, the host immune response has to be prevented. This study was performed in order to analyze the mechanisms and dynamics of neural xenograft rejection, as well as neurobiological properties of the donor tissue. Adult normal
Mitochondrial biogenesis and function in plants require the expression of over 1000 nuclear genes encoding mitochondrial proteins (NGEMPs). The expression of these genes is regulated by tissue-specific, developmental, internal, and external stimuli that result in a dynamic organelle involved in both metabolic and a variety of signaling processes. Although the metabolic and biosynthetic machinery o
The expression of a variety of nuclear genes encoding mitochondrial proteins is known to adapt to changes in environmental conditions and retrograde signaling. The presence of putative WRKY transcription factor binding sites (W-boxes) in the promoters of many of these genes prompted a screen of 72 annotated WRKY factors in the Arabidopsis (Arabidopsis thaliana) genome for regulators of transcripts
We used PCR and DNA sequencing to screen for haemosporidian parasites (Haemoproteus and Plasmodium) in 2,488 individual birds from 104 species and 22 families, primarily understory suboscine passerines, captured in a lowland Amazonian forest in Ecuador as a first major step to understanding the transmission dynamics of this cosmopolitan group of parasites in this region. To assess diversity of avi
Glycoproteins in cerebrospinal fluid (CSF) are altered in Alzheimer's Disease (AD) patients compared to control individuals. We have utilized albumin depletion prior to 2D gel electrophoresis to enhance glycoprotein concentration for image analysis as well as structural glycoprotein determination without glycan release using mass spectrometry (MS). The benefits of a direct glycoprotein analysis ap
Genes and proteins are known to have differences in their sensitivity to alterations. Despite numerous sequencing studies, proportions of harmful and harmless substitutions are not known for proteins and groups of proteins. To address this question, we predicted the outcome for all possible single amino acid substitutions (AASs) in nine representative protein groups by using the PON-P2 method. The
Quantitative proteomics plays an important role in validation of breast-cancer-related biomarkers. In this study, we systematically compared the performance of label-free quantification (LFQ) and SILAC with shotgun and directed methods for quantifying breast-cancer-related markers in microdissected tissues. We show that LFQ leads to slightly higher coefficient of variation (CV) for protein quantif
The 2-oxoglutarate dehydrogenase complex consists of three different subenzymes, the E1o (2-oxoglutarate dehydrogenase) component, the E2o (dihydrolipoyl transsuccinylase) component, and the E3 (dihydrolipoamide dehydrogenase) component. In Bacillus subtilis, the E1o and E2o subenzymes are encoded by odhA and odhB, respectively. A plasmid with a 6.8-kilobase-pair DNA fragment containing odhA and o
Phenotypic variance heterogeneity across genotypes at a single nucleotide polymorphism (SNP) may reflect underlying gene-environment (G×E) or gene-gene interactions. We modeled variance heterogeneity for blood lipids and BMI in up to 44,211 participants and investigated relationships between variance effects (Pv), G×E interaction effects (with smoking and physical activity), and marginal genetic e
Antimicrobial peptides (AMPs) provide a first line of defense against bacterial infections. Here we report that urine levels of AMPs, locally produced in the urinary tract, are lower in individuals with asymptomatic bacteriuria (ABU) compared to patients with urinary tract infection (UTI).
The impact of thyroid surgery on voice is a key performance indicator of surgical quality. This chapter discusses factors that can impact on the incidence of both recurrent and external laryngeal nerve (external branch of superior laryngeal nerve-EBSLN) injury. The literature is reviewed and reported incidence of nerve palsy following thyroid surgery is presented. Particular reference is made to t
This thesis deals with cerebral arterial vasospasm and ischaemia, a serious complication after aneurysmal subarachnoid haemorrhage. Firstly is the noninvasive transcranial Doppler ultrasound and transcranial cerebral oximetry techniques evaluated in detecting cerebral arterial vasospasm in clinical practice. Patients were examined during first 14 days after the bleed. Flow velocities in normo- ver
Methods for monitoring seepage and detecting internal erosion are essential for the safety evaluation of embankment dams. Internal erosion is one of the major reasons for embankment dam failures, and there are several tenths of thousands of large embankment dams in the world. Internal erosion is progressing inside the dam, and is sometimes difficult to detect by conventional methods. Therefore, th
Popular Abstract in Swedish Optoelektronik genomgår för närvarande en otrolig utveckling, inte minst på grund av de senaste årtiondenas kommersialisering och utveckling av hemelektronik som kompaktdiskar och digitalkameror. Denna utveckling har drivit en blomstrande global tillväxt för optoelektroniska företag som varje år utvidgar sina erbjudanden av optiska komponenter i hård konkurrens. UtvecklThe present thesis describes a number of aspects of modern electro-optical measurement technology also known as bio-photonics; this includes instrumentation, applications, sample interaction and data interpretation. The methods employed operate over several domains, and light measurements are discretized both in intensity, space, angle, time, polarization and energy. Mainly the spectral domain is
Popular Abstract in Swedish Kroppens rörelser åstadkommes genom sammandragning av muskelvävnad som kan vara av två slag, glatt eller tvärstrimmig. Den senare sorten kan indelas i skelett och hjärtmuskulatur, vilka har mycket olika egenskaper trots den gemensamma benämningen. Muskelvävnaden är uppbyggd av långsträckta, spolformade celler som innehåller tunna och tjocka kontraktila filament, beståenThe initial signal for contraction in smooth muscle is an increase in [Ca2+]i. Ca2+ ions can either enter the cytosol from the extracellular medium or be released from intracellular stores. Voltage-operated Ca2+ channels (VOC) play a dominant role in the activation of smooth muscle contraction. This thesis describes two novel regulatory mechanisms of the dihydropyridine (DHP)-sensitive VOC in smoo
