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PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. Among the mechanisms by which these inhibitors may act are through interfering wi

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Context: With new astronomical infrared spectrographs the demands of accurate atomic data in the infrared have increased. In this region there is a large amount of parity-forbidden lines, which are of importance in diagnostics of low-density astrophysical plasmas. Aims: We present improved, experimentally determined, energy levels for the lowest even LS terms of Fe ii, Ti ii and Cr ii, along with

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Popular Abstract in Swedish Glukos är kroppens viktigaste energikälla och distribueras via blodet. Hjärncellerna är helt beroende av glukos för sin energiförsörjning. Nervceller har dålig förmåga att lagra glukos och låga blodsockerhalter påverkar negativt dessa cellers funktion och kan ytterst leda till koma och död. Det är därför inte förvånande att blodsockerhalten normalt hålles inom ganska snAbstract The electrophysiological properties of insulin-secreting b-cells, glucagon-releasing a-cells and somatostation-producing d-cells in intact mouse pancreatic islets were investigated using the perforated patch whole-cell technique. It is demonstrated that it is possible to functionally identify the different cell types in situ. The ?-cells have a cell capacitance of 7.4 pF and differ from

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Calculation of relative humidity and temperature in an early stage of the design process is important to avoid moisture damages in wooden walls. Previous studies show that a sufficient air flow in the air gap behind the façade panel is important to ensure a moisture safe construction. This study investigates if blind WUFI 5.0 calculations with a varied air flow in the air gap behind the façade giv

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Aim This paper seeks to investigate whether alpine floras on isolated mountains in boreal forest show nestedness, and, if that is the case, to determine whether selective extinction or colonization is the likely cause of the observed patterns. Location Isolated mountains in the boreal coniferous forests of northern Sweden (province of Norrbotten, c. 66°N; 18°E). The timberline in the region probab

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Cellulose is one of the most abundant biopolymers on earth. Several organisms degrade cellulose, one of the most studied is the fungus Trichoderma reesei which produces seven genetically different cellulases. In this thesis, a thorough study of the hydrolytic characteristics of the less studied endoglucanases Cel12A (EG III), Cel45A (EG V) and Cel61A (EG IV) of T. reesei is presented

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This paper discusses the effects of cooled EGR on a turbocharged multi-cylinder HCCI engine. A six-cylinder, 12-liter, Scania D12 truck engine is modified for HCCI operation. It is fitted with port fuel injection of ethanol and n-heptane and cylinder pressure sensors for closed-loop combustion control. The effects of EGR are studied in different operating regimes of the engine. During idle, low sp

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OBJECTIVE: to explore Sudanese midwives' motives for and perceptions and experiences of re-infibulation after birth and to elucidate its context and determinants. DESIGN: triangulation of methods, using observational techniques and open-ended interviews. SETTING AND PARTICIPANTS: two government hospitals in Khartoum/Omdurman, Sudan, for the observations and in-depth interviews with 17 midwives. FI

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In this report, we provide a perspective on how zooplankton are able to respond to present and future levels of ultraviolet (UV) radiation, a threat that has been present throughout evolutionary time. To cope with this threat, zooplankton have evolved several adaptations including behavioral responses, repair systems, and accumulation of photoprotective compounds. Common photoprotective compounds

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Enteric neuronal plasticity is probably fundamental in order to withstand injury or changes in intestinal activity. The role of the neuropeptides in neuroprotection is still enigmatic. The expression of galanin and vasoactive intestinal peptide (VIP) and the effects of the two peptides on survival of small intestinal porcine myenteric neurons cultured for 6 days were studied. Immunocytochemistry a

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Aims: Two common reasons to explain slow environmental biodegradation of polycyclic aromatic hydrocarbons (PAHs), namely lack of appropriate carbon sources for microbial growth and limited bioavailability of PAHs, were tested in a laboratory bioassay using a creosote-contaminated soil. Methods and Results: The soil, containing a total of 8 mg g(-1) of 16 PAHs, was sieved and incubated in bottles f

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The near-edge fine structure of the Fe2p and Si2p X-ray absorption spectra (NEXAFS) of iron-oxygen nanolayers on the surface of single-crystal silicon (100) was studied for the first time. The structure of the quasi-2D iron-oxygen nanosystems synthesized by molecular layering was considered on this basis.

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The performance of an anaerobic digestion process is much dependent on the type and the composition of the material to be digested. The effects on the degradation process of co-digesting different types of waste were examined in two laboratory-scale studies. In the first investigation, sewage sludge was co-digested with industrial waste from potato processing. The co-digestion resulted in a low bu