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The activity of violaxanthin de-epoxidase has been studied both in isolated thylakoids and after partial purification, as a function of pH and ascorbate concentration. We demonstrate that violaxanthin de-epoxidase has a Km for ascorbate that is strongly dependent on pH, with values of 10, 2.5, 1.0 and 0.3 mM at pH 6.0, 5.5, 5.0 and 4.5, respectively. These values can be expressed as a single Km±0.
Cationic surfactants associate strongly to DNA and compact but are often toxic. The interaction of some novel cationic amino acid-based surfactants, which may enhance transfection and appear to be nontoxic, is described. A cationic arginine-based surfactant, ALA, gives in combination with anionic surfactants spontaneously stable vesicles, and special attention is given to the association of these
Aims The main aim of this study was to describe the age at which pathological aortic root dilation occurs in patients with Marfan's syndrome (MFS). Methods and results A total of 160 patients with MFS attending a regional cardiac centre were reviewed retrospectively. Dilation of the ascending aorta was diagnosed by comparing the maximum aortic sinus measurement with control data from the literatur
Simultaneous SO2 and NOx reduction in flue gas by the dry furnace sorbent injection process was simulated with an entrained-flow reactor. Several ammonium salts as well as urea were tested. Urea was found to give the highest NOx removal efficiency. By using urea-limestone sorbent, >90% SO2 removal and >80% NOx removal were obtained at Ca/S and N/NOx ratios both equal to 2. The DeNO(x) process is m
This working paper discusses the different Asian experiences of the Green Revolution from the late 1960s to the 1980s in the perspective of earlier developments of agricultural intensification. The intention of the paper is to identify common features and conditions contributing to the considerable success of Asian agriculture over the past 30 years. The authors argue that the Green Revolution mus
A series of structures of trans-[MX(2)(PBz(3))(2)] [M = Pt, X = Cl(-); PBz(3) = tribenzylphosphine (1), I(-), trans-diiodobis(tribenzylphosphine)platinum(II) (2), and NCS(-), trans-di(thiocyanate)bis(tribenzylphosphine)platinum(II) (3); M = Pd, X = I(-), trans-diiodobis(tribenzylphosphine)palladium(II) (4)] have been characterized by X-ray crystallography. In all compounds each tribenzylphosphine
Glucose-evoked insulin secretion exhibits two phases: The first phase represents exocytosis of insulin granules situated at the plasma membrane, whereas the second phase reflects insulin release from granules continuously recruited to the plasma membrane. This thesis investigates different aspects of phasic insulin secretion, with the specific aims to elucidate the possible contribution of insulin
The influence of pH on membranes of different materials and different cut off has been studied. One ceramic microfiltration membrane and three synthetic ultrafiltration membranes were included in the study. The pH of an acidic (C-stage) and and alkaline (E-stage) bleaching effluent was varied. For the E-stage effluent the membrane performance seemed to be correlated to the cut off of the membrane.
An integrated view on the possible effects of global climate change is provided while taking into account that not only the rising average temperature is likely to impact natural populations but also that increased variation around the mean and higher frequency of extreme events will be important. We propose that complex genetic effects in concert with demographic patterns may affect how focal pop
This thesis is focused on natural killer (NK) T cells. NKT cells recognize lipids and glycolipids, rather than peptides, in the contents of the antigen presenting molecule CD1d. NKT cells have a surface phenotype reminiscent of memory cells, and have been shown to rapidly produce large amounts of cytokines, such as IL-4 and IFN-gamma, upon activation. Because of their rapid response to activation,
Gamma rays in the N=Z + 1 nucleus Y-79 were identified using the reaction Si-28(Fe-54, p2n)Y-79 at a 200 MeV beam energy and an experimental setup consisting of an array of Ge detectors and the Recoil Mass Spectrometer at Oak Ridge National Laboratory. With the help of additional gamma-gamma coincidence data obtained with Gammasphere, these gamma rays were found to form a strongly coupled rotation
The aim of this thesis was to prospectively evaluate the short-term effect of a non-steroidal anti-inflammatory drug (diclofenac sodium, Voltaren) and stabilisation appliance in controlled trials of patients with temporomandibular disorders (TMD) of arthrogeneous origin. The active treatments were compared to placebo in the study of diclofenac and to a control appliance in the other study. Thirty-
The radioanalytical procedures for the determination of neutron activation isotopes (Fe-55 and Ni-63) in environmental samples (water, sediment, sail and biological material) are described. The procedures are based on the coprecipitation of Fe-55 with iron hydroxide in natural and reactor water, ashing and mineralization of sediments, soil and biota samples, and the sequential separation and purif
The Homogenous Charge Compression Ignition (HCCI) combustion engine has potential to replace the spark ignition and compression ignition engines of today. One of the main problems in making the engine commercially attractive is the lack of direct means of controlling the ignition phasing. In this paper, we investigate the potential of inlet air temperature as a means to ignition actuation. This ar
It has been proposed that excessive and uncontrolled proteolytic activity is an important pathogenetic factor for chronic wounds. Identification of molecules that either control or reflect proteolysis in wounds may prove to be useful in determining wound healing activity. In this study wound fluid was sampled under a polyurethane dressing or on hydrophilic glass filters. Multiple chronic wound flu
