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DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B rad
The influence of temperature and salt concentration on the properties of solid polymer electrolytes based on lithium bis(trifluoromethylsulfonyl)imide (LiTFSI) complexed by combshaped polyethers has been studied. The combshaped polyethers consisted of styrenic backbone polymers carrying pendant poly(ethylene oxide) (PEO) or poly(ethylene oxide-co-propylene oxide) (PEOPO) side chains terminated by
Background: The co-morbidity of cystic fibrosis (CF) and celiac disease (CD) has been reported sporadically since the 1960s. To our knowledge, this is the first time a systematic screening is performed in a large cohort of CF patients. Methods: Transglutaminase-IgA (TGA), endomysium-IgA (EMA) and total IgA in serum were measured in 790 CF patients (48% females, 86% with pancreatic insufficiency).
A study was undertaken to measure changes in oxygen consumption and metabolite concentration (ammonia, free amino acids, glucose, glycogen, ninhydrin positive substances and protein) in embryos of yellowtail kingfish (Seriola lalandi) incubated at different temperatures (17, 19, 21 and 23 °C). The oxygen uptake at hatch and total oxygen consumed during embryogenesis was negatively correlated with
Multiconfigurational quantum chemical calculations on the R-diimines dichromium compound confirm that the Cr-Cr bond, 1.80 angstrom, is among the shortest Cr-I-Cr-I bonds. However, the bond between the two Cr atoms is only a quadruple bond rather than a quintuple bond. The reason why the bond is so short has to be attributed to the strain in the NCCN ligand moieties.
Introduction: Different sources of variations, such as electrogoniometer characteristics and procedures, may affect the accuracy and precision of movement measurements during gait. Objective: To quantify the variations and compare the effects produced by different sources of variation in electrogoniometric gait recordings: the sensors, procedures (trials) and the knee joint. Methods: Knee flexion/
To estimate the burden of disease due to congenital toxoplasmosis in Sweden the incidence of primary infections during pregnancy and birth prevalence of congenital toxoplasmosis in 40978 children born in two regions in Sweden was determined. Women possibly infected during pregnancy were identi®ed based on: 1, detection of speci®c IgG based on neonatal screening of the phenylketonuria (PKU) card bl
The hole - vibrational coupling in naphthalene is studied using high-resolution gas-phase photoelectron spectroscopy and density functional theory calculations (DFT), and a remarkable increase of the coupling with low-frequency vibrations is observed in the excited states.
An assay has been established for quantitative evaluation of lentivirus coreceptor use with the help of GHOST(3) cells. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, CXCR6/STRL33/Bonzo, or the orphan receptor GPR15/BOB. The indicator cell line carries the HIV-2
The enzymes needed for galactomannan hydrolysis, i.e. β-mannanase, α-galactosidase and β-mannosidase, were produced by the filamentous fungus Aspergillus niger. The β-mannanase was purified to electrophoretic homogeneity in three steps using ammonium sulfate precipitation, anion-exchange chromatography and gel filtration. The purified enzyme had an isoelectric point of 3.7 and a molecular mass of
Many fish depend primarily on their tail beat for propulsion. Such a tail is commonly modeled as a two-dimensional flapping foil. Here we demonstrate a novel experimental setup of such a foil that heaves and pitches in a soap film. The vortical flow field generated by the foil correlates with thickness variations in the soap film, which appear as interference fringes when the film is illuminated w
The role of sphingosine kinase (SphK) on basic fibroblast growth factor (bFGF)-induced proliferation of cerebral, aortic and coronary smooth muscle cells (SMC) was addressed using D-erythro-N,N-dimethylsphingosine (DMS), an inhibitor of SphK which blocks conversion of sphingosine to sphingosine-1-phosphate (S1P). DMS concentration-dependently reduced the bFGF-induced proliferation of rat cerebral
The traditional design method for plate heat exchangers(PHEs), either epsilon-number of transfer units (epsilon-NTU) or logarithmic mean temperature difference method, involves many trials in order to meet the pressure drop constraints. This can be avoided through the developed design method, which takes the full utilization of the allowable pressure drops as a design objective. The proposed metho
Human cystatins C and D share almost identical primary structures of two out of the three segments proposed to be of importance for enzyme interactions but have markedly different profiles for inhibition of the target cysteine peptidases, cathepsins B, H, L, and S. To investigate if the N-terminal binding regions of the inhibitors are responsible for the different inhibition profiles, and thereby
Beta-hexosaminidase activity in plasma and urine was measured and compared in insulin dependent diabetics (IDDM) with and without proliferative retinopathy. No difference in the activity of beta-hexosaminidase was found between the two groups indicating that this enzyme is not involved in the development of diabetic microangiopathy.
A follow-up study was performed to investigate the stability of IQ measures in a group of dyslexic teenagers and young adults. Earlier research had shown contradictory results. The 65 subjects, 12 years old on the average at first test, were retested after a mean interval of six and a half years. There was a significant relative decrease in verbal IQ (VIQ), which was interpreted as either an effec
In the process of making ethanol from lignocellulosic materials, compounds inhibitory to microorganisms are generated during steam pretreatment of the wood. Water-soluble inhibitors and pentoses are liberated and washed from the cellulose structure which is further enzymatically hydrolyzed. To make the process economically feasible, rite pentoses have to be fermented to ethanol. A major drawback w
