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AIM: To study the interaction between the human cysteine proteinase inhibitor, cystatin C, and proteinases of periodontitis associated bacteria. METHODS: Gingival crevicular fluid samples were collected from discrete periodontitis sites and their cystatin C content was estimated by enzyme linked immunosorbent assay (ELISA). The interaction between cystatin C and proteolytic enzymes from cultured s
Magnetotransport in GaAs/AlAs/GaAs single-barrier heterostructures, incorporating unintentional donors in the barrier, is studied. Resonant tunneling is observed through the quasiconfined states in the AlAs layer which originate from the X-XY and X-Z conduction-band minima and through two distinct states of the donors bound to the X-XY and X-Z valleys. This allowes us to determine directly the bin
NADH:quinone oxidoreductase, or Complex I, is a multi-subunit membrane-bound enzyme in the respiratory chain of many pro- and eukaryotes. The enzyme catalyzes the oxidation of NADH and donates electrons to the quinone pool, coupled to proton translocation across the membrane, but the mechanism of energy transduction is not understood. In bacteria the enzyme consists of 14 subunits, seven membrane
-Glucosidases belong to families 1, 3 and 9 of the glycoside hydrolases and act on cello-oligosaccharides. Family 1 and 3 enzymes are retaining and are reported to have transglycosylation activity, which can be used to produce oligosaccharides and glycoconjugates. Family 3 enzymes are less well characterized than their family 1 homologues and to date only two crystal structures have been solved. H
Prostaglandin E1 has earlier been shown to decrease pulmonary platelet trapping (PPT) following shock. This experiment was performed to evaluate a new method to study PPT in vivo, and to study the effect of prostaglandin E1 and a new antiplatelet drug (ticlopidine) on PPT in rabbits after i.v. administration of endotoxin. Following platelet labeling with In-111, the rabbits were placed under a sci
The Saccharomyces cerevisiae leukotriene A4 (LTA4) hydrolase (scLTA4 hydrolase) has been crystallized in order to study the two activities of LTA4 hydrolase in an evolutionary perspective. Single well diffracting crystals are obtained after switching from the hanging-drop method to liquid-liquid diffusion in capillaries using PEG 8000 as precipitant. These crystals belong to space group P212121, w
Popular Abstract in Swedish De flesta primärar hjärntumörer utgår ifrån astrocyter, oligodendrocyter och ependymala celler, som är hjärnvävnadens egna celler. Cirka hälften av dessa tumörer ingår i en grupp där tumörtypen heter gliom. Glioblastoma multiforme (GBM) är den mest elakartade tumören inom denna grupp och också den vanligast förekommande.Trots stora forskningssatsningar är behandlingsstrGlioblastoma multiforme (GBM) is the most malignant and also the most common form of brain tumour in humans. The overall aim of this thesis was to investigate morphological and diagnostics features in human GBM that may be of importance for prognosis and treatment. Immunohistochemically stained tissue sections have been microscopically analysed regarding immuno-reactive cells, cell proliferation,
A new way of preparing catalytically active wire meshes through a thermal-spray technique is described. A metal substrate (e.g. Kanthal AF) was plasma-sprayed with a composite ceramic/polymer-powder. The polymer content of the sprayed layer was burnt off whereupon a well-defined macro-porosity was created. By treating the so obtained material with an alumina-sol the specific surface area could be
In order to avoid environmental threats, organisms may respond by altering behavior or phenotype. Using experiments performed in high-latitude Siberia and in temperate Sweden, we show for the first time that, among freshwater crustacean zooplankton, the defense against threats from ultraviolet radiation (UV) is a system where phenotypic plasticity and behavioral escape mechanisms function as compl
The present study focuses on the preparation of poly(ethylene oxide) (PEO) nanocomposites based on the synthetic Laponite clay. The clay was added both in its pure form as well as organically modified with low molar mass poly(ethylene glycol) (PEG) components in order to enhance the compatibility between Laponite and PEO. Several PEG's with different end groups were used. Almost all of them were f
Trichoderma cellulases appear in several isoforms which makes their purification and analysis difficult. We used fast protein liquid chromatography (FPLC) to purify three major cellulases and to quantitate these enzymes in reconstituted mixtures during cellulose hydrolysis studies (in lack of specific substrates and because of the synergism between the enzymes such analysis is very difficult, if a
Many promising robotics research results were obtained during the late 1970s and early 1980s. Some examples include Cartesian force control and advanced motion planning. Now, 20 years and many research projects later, many technologies still have not reached industrial usage. An important question to consider is how this situation can be improved for future deployment of necessary technologies. To
This paper investigates the impact of labour-market attachment on first births of foreign-born women in Sweden. The study uses a longitudinal, register-based dataset consisting of the entire population of immigrants from ten nations and a 5-per-cent random sample of natives. The effects of earned income are evident, with increased income levels increasing the probability of becoming a mother for a
The intestinal mucosa represents the largest body surface toward the external environment and harbors numerous T lymphocytes that take up resident within the intestinal epithelium or in the underlying lamina propria (LP). The intraepithelial lymphocytes include subsets of 'unconventional' T cells with unclear ontogeny and reactivity that localize to this site independently of antigen-specific acti
Abstract: Previous experiments have shown that severe hypoglycemia disrupts cerebral energy state in spite of a maintained cerebral oxygen consumption, suggesting uncoupling of oxidative phosphorylation. Other studies have demonstrated that hypoglycemia leads to loss of cerebral cortical phospholipids and phospholipid-bound fatty acids. The objective of the present study was, therefore, to study r
A liquid-crystalline nanoparticles with internal reversed hexagonal structure has been prepared by use of a single lipid system. The obtained particles are essentially monocrystalline nanoporous hexagonal prisms with the size of about 200 nm.
