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Calbindin D-28k, a highly conserved protein with Ca2+-sensing and Ca2+-buffering capabilities, is abundant in brain and sensory neurons. This protein contains six EF-hand subdomains, four of which bind Call with high affinity. Calbindin D28k can be reconstituted from six synthetic peptides corresponding to the six EF-hands, indicating a single-domain structure with multiple interactions between th

Cas12e proteins (formerly CasX) form a distinct subtype of Class II type V CRISPR-Cas effectors. Recently, it was shown that DpbCas12e from Deltaproteobacteria and PlmCas12e from Planctomycetes can introduce programmable double-stranded breaks in mammalian genomes. Thus, along with Cas9 and Cas12a Class II effectors, Cas12e could be harnessed for genome editing and engineering. The location of cle

Long-term tall-tower eddy-covariance (EC) measurements have been recently established in three European pilot cities as part of the ICOS-Cities project. We conducted a comparison of EC software to ensure a reliable generation of interoperable flux estimates, which is the prerequisite for avoiding methodological biases and improving the comparability of the results. We analyzed datasets covering 5

Policy intervention schemes for increased deployment of solar photovoltaics (PV) have been launched in several countries, with varying success. In order to ensure an efficient deployment of the technology, thorough knowledge is needed about relevant present actors and institutions, and about how a desirable actor base and institutional setup should look like; mere cost reductions are not enough to

Where and how new industrial paths emerge are much debated questions in economic geography, especially in light of the recent evolutionary turn. This article contributes to the ongoing debate on path creation with a new analytical framework that specifies the formation of generic resources in embryonic industries. It suggests that path creation processes are not only conditioned by preexisting reg

Plant cells have two main modes of growth generating anisotropic structures. Diffuse growth where whole cell walls extend in specific directions, guided by anisotropically positioned cellulose fibers, and tip growth, with inhomogeneous addition of new cell wall material at the tip of the structure. Cells are known to regulate these processes via molecular signals and the cytoskeleton. Mechanical s

Objective: To study whether locally administered recombinant inactivated human coagulation factor VIIa (FFR-rFVIIa) would reduce the thrombus formation and improve patency in an experimental venous thrombosis model without inducing systematic changes in the coagulation. Design: Experimental double-dummy randomised study. Materials: In 20 healthy New Zealand White rabbits both juguIar veins were ex

Nigritella miniata is an alpine orchid species disjunctly endemic to the Eastern Alps and the Southeastern Carpathians. It is thought to have arisen only once through a hybridisation event between now extinct ancestors. Questions of interest are whether N. miniata survived in few or many isolated refugia in the Eastern Alps during the Last Glacial Maximum (LGM), and their relationship with the pop

As China modernizes, its waste management capacities are put to a test. Faced by significant pressures to find a solution for growing municipal solid waste, China’s authorities have embraced waste incineration technology as a seemingly cost-efficient alternative to landfills. Against this push, significant societal opposition against the construction of waste incinerators has been mounting. Yet wh

Popular summary Energy generation from renewable sources is one of the main issues to be solved for the 21st century. One of the most advanced technologies currently available is wind energy, which has a large potential onshore and offshore. However, in many cases wind energy is restricted to large wind parks with wind power plants sometimes exceeding heights of 100 m. The construction of large wi

Seven or eight thousand years ago, as the farthest the human memory can reach, the Egyptians established a great civilization that was considered as the origin of civilization on the Earth. There is no place in Egypt where man cannot find evidence that enlighten this civilization and tell about Egypt's role in most of the world's historic events from the beginning of mankind until the pres

Understanding the spatiotemporal distribution of birds is crucial for effective management and conservation of their populations. However, we still have only limited knowledge not only of the wintering destinations of many Iberian breeding migrants but also of aspects as general as the migratory behaviour of the populations of many common avian species that breed in the Iberian highlands. We used