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Major businesses today involves complex supply chains with world-wide sourcing and marketing which create new risks for both the individual enterprise and the society at large. Supply chain disruptions are seen as the primary threat to a company's revenue driver. To be able to cope with undesired events, companies need to review their business continuity management (BCM). To increase the know
A computational method is described and tested for prediction of ligand-binding poses between the human farnesoid X receptor and a set of 36 potential agonists, provided by the D3R Grand Challenge 2016. Using tools such as Molecular Docking, Molecular Dynamics, Reconnaissance metadynamics and cluster analysis, the method is an attempt to predict the binding pose without being biased by experimenta
Understanding the genetic regulation of blood CD34+ cell levels could lead to better methods for stem cell mobilization and transplantation. Recently, in a genome-wide association study (GWAS), we identified 11 genetic associations with blood CD34+ cell levels. One of the most significant maps to an intergenic region between the ENO1 and RERE genes at chromosome 1p36.23. However, the underlying me
Boreal forests play a crucial role in the global carbon cycle and currently act as an important carbon sink. At the same time, these forests are increasingly affected by intensive forest management and a changing disturbance regime driven by climate change. Clear-cutting has been the dominant harvesting method in managed boreal forests, while wildfire, although rare over the last century in Sweden
Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in the circular visceral mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify Alk targets in embryos
Somatic hypermutation and isotype switch recombination occur in germinal center B cells, are linked to transcription, and are similarly affected by deficiency in MutS homologue (MSH)2. Class-switch recombination is abrogated by disruption of genes encoding components of the catalytic subunit of DNA-dependent protein kinase (DNA-PK(cs))/Ku complex and likely involves nonhomologous end joining (NHEJ
Bacterial exotoxins with ADP-ribosyltransferase activity can be divided into distinct clades based on their domain organization. Exotoxins from several clades are known to modify actin at Arg177; but of the 14-3-3 dependent exotoxins only Aeromonas salmonicida exoenzyme T (AexT) has been reported to ADP-ribosylate actin. Given the extensive similarity among the 14-3-3 dependent exotoxins, we initi
Serialization is an old narrative strategy and a form of publication that can be traced far back in literary history, yet serial narratives are as popular as ever. This book investigates a resurgence of serial narratives in contemporary literary culture.Analyzing series as diverse as Mark Z. Danielewski’s experimental book series The Familiar; audiobook series by the Swedish streaming service Stor
Triosephosphate isomerase (TIM) is a key enzyme in glycolysis that catalyses the interconversion of glyceraldehyde 3-phosphate and dihydroxy-acetone phosphate. This simple reaction involves the shuttling of protons mediated by protolysable side chains. The catalytic power of TIM is thought to stem from its ability to facilitate the deprotonation of a carbon next to a carbonyl group to generate an
Urban renewal is a process that every city will inevitably go through, but how to retain the historical values in the renewal? Gaoqiao town is a historical and cultural town in Pudong New Area, Shanghai. The history of houses has more than 80 years old. In 2001, the “One City Nine Towns Development Plan” was introduced in Shanghai. The Pudong New Area government aims to develop Gaoqiao Town into a
Groups of male mice were exposed via inhalation to methylene chloride, perchloroethylene, toluene, trichloroethylene or 1,1,1-trichloroethane. The exposures were started at 2300 h. Generation of vapor was stopped after 1 h. Motor activity of the animals during the exposures was measured with a Doppler radar. Several concentrations of each solvent were tested. Concentrations could be found for all
Cystatin C (CstC) is a potent cysteine-proteinase inhibitor. The structure of the mouse CstC-encoding gene (Cst3) was examined by sequencing a 6.1-kb genomic DNA containing the entire gene, as well as 0.9 kb of 5′ flanking and 1.7 kb of its 3′ flanking region. The sequence revealed that the overall organization of the gene is very similar to those of the genes encoding human CstC and other type-2
L-3,4-dihydroxyphenylalanine (L-DOPA) remains the most efficacious drug for the treatment of Parkinson's disease (PD), but causes adverse effects that limit its utility. L-DOPA-induced dyskinesia (abnormal involuntary movements) is a significant clinical problem that attracts growing scientific interest. Current notions attribute the development of dyskinesia to two main factors, viz. the loss of
