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The intracellular localization of soluble and membrane-bound isoforms of rat and human catechol O-methyltransferase (COMT) was studied by expressing the recombinant COMT proteins either separately or together in mammalian cell lines (HeLa and COS-7 cells) and in rat primary neurons. The distribution of soluble and membrane-bound COMT enzyme was visualized by immunocytochemistry. For comparison, th
Previous biochemical and histochemical studies have suggested that catechol-O-methyltransferase (COMT) is a predominantly glial enzyme in the brain. The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily distinguished with specific markers. COMT immunoreactivity was studied in primary astrocytic cultures from newborn rat cerebral
In Saccharomyces cerevisiae, pH homeostasis is reliant on ATP due to the use of proton-translocating ATPase (H+-ATPase) which constitutes a major drain within cellular ATP supply. Here, an exogenous proton-translocating pyrophosphatase (H+-PPase) from Arabidopsis thaliana, which uses inorganic pyrophosphate (PPi) rather than ATP, was evaluated for its effect on reducing the ATP burden. The H+-Ppas
This thesis presents a study of the interplay between what numbers do and what people do with numbers in everyday organisational life. Couched in an ethnographic perspective, the study draws on rich empirical material crafted from participant observations conducted at a Scandinavian hospital. The study focuses on how numbers work in everyday social interactions between doctors, nurses, patients, a
The present study seeks to expand the current focus on acquisition situations in linguistic relativity research by exploring the effects of nativisation (the process by which a L2 is acquired as a L1) on language-specific cognitive behaviour. Categorisation preferences of goal-oriented motion events were investigated in South African speakers who learnt English as a L1 from caregivers who spoke En
The production of η and η′ mesons is studied in proton-proton and proton-lead collisions collected with the LHCb detector. Proton-proton collisions are studied at center-of-mass energies of 5.02 and 13TeV and proton-lead collisions are studied at a center-of-mass energy per nucleon of 8.16TeV. The studies are performed in center-of-mass (c.m.) rapidity regions 2.5
Background and purpose: Pyruvate dehydrogenase complex deficiency is in up to 90% caused by pathogenic variants in the X-linked PDHA1 gene. We aimed to investigate female relatives of index patients with PDHA1-related disease to (i) describe the prevalence of female PDHA1 carriers, (ii) determine whether they had symptoms and signs, and (iii) delineate the associated phenotype. Methods: In a natio
This paper introduces the MinCan database that presents mine-level estimates for the Canadian mining industry with a persistent annual coverage between 1950 and 2022. These estimates are based on archival maps and a selection of historical sources, which follows a hierarchy of criteria-based credibility and standardization. The information contained in MinCan covers 947 mines and provides informat
In this paper, curve-fitting and an artificial neural network (ANN) model were developed to predict R-Event. Expected number of new infections that arise in any event occurring over a total time in any space is termed as R-Event. Real-time data for the office environment was gathered in the spring of 2022 in a naturally ventilated office room in Roorkee, India, under composite climatic conditions.
The remodelling process in bones is closely related to electromechanical phenomena. In addition to streaming potentials and piezoelectricity, flexoelectricity has been found to serve as an initiator for remodelling in cortical bone. Since flexoelectricity is coupled to strain gradients, the effect is size-dependent and, accordingly, most relevant on small scales. This means that particularly micro
Earlier this year, authorities in the Xinjiang Uyghur Autonomous Region passed a revised version of the Xinjiang Regulations on Religious Affairs. The new rules emphasize the Sinicization of religion, ensuring that sites of religious worship, like mosques, reflect “Chinese characteristics and style.” Moreover, they are calling for the cultivation of “patriotic” religious leaders. Despite enshrinin
Childhood cancer survivors (CCS) require specialized follow-up throughout their lifespan to prevent or manage late effects of cancer treatment. Knowing the size and structure of the population of CCS is crucial to plan interventions. In this scoping review we reviewed studies that reported prevalence of CCS in Europe. We searched Medline, Web of Science, and Embase using permutations of terms refe
Electroactive polymers (EAPs) deform when subject to an electric field, which is generated by two or more electrodes. To ensure proper function of the EAP, these electrodes are connected to a source and they are therefore required to be continuous such that no isolated islands exist. Increasing an EAP’s performance using topology optimization while ensuring electrode connectivity is the goal of th
Reaction products in heterogeneous catalysis can be detected either on the catalyst surface or in the gas phase after desorption. However, if atoms are dissolved in the catalyst bulk, then reaction channels can become hidden. This is the case if the dissolution rate of the deposits is faster than their formation rate. This might lead to the underestimation or even overlooking of reaction channels
Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated pa
